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首页> 外文期刊>Journal of Veterinary Diagnostic Investigation >Phenotypic and genotypic characterization of antimicrobial resistance in enterohemorrhagic Escherichia coli and atypical enteropathogenic E. coli strains from ruminants.
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Phenotypic and genotypic characterization of antimicrobial resistance in enterohemorrhagic Escherichia coli and atypical enteropathogenic E. coli strains from ruminants.

机译：肠出血性大肠杆菌和反刍动物的非典型肠致病性大肠杆菌菌株的抗菌素耐药性的表型和基因型特征。

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摘要

Two hundred and twenty-six attaching and effacing Escherichia coli (AEEC) strains (20 enterohemorrhagic E. coli and 206 atypical enteropathogenic E. coli) isolated from calves, lambs, and goat kids with diarrhea and from healthy cattle, sheep, and goats were tested for their resistance to 10 antimicrobial agents by the disc diffusion method. Resistant and intermediate strains were analyzed by polymerase chain reaction for the presence of the major resistance genes. The overall percentage of resistant strains to tetracycline, streptomycin, erythromycin, and sulfamethoxazole was very high (>65%). Moreover, a high level of resistance (approximately 30%) to ampicillin, chloramphenicol, trimethoprim, and trimethoprim-sulfamethoxazole was also detected. The AEEC strains were very susceptible (>90%) to gentamicin and colistin. Because AEEC from ruminants can cause diseases in human beings, the high frequency of antimicrobial resistance detected in the current study is a source of concern. For each antimicrobial agent, the predominant resistance genes in the resistant strains were ampicillin, bla(TEM) (97.1%); tetracycline, tetA (76.7%); gentamicin, aac(3)II (80%); streptomycin, strA/strB (76.7%) and aadA (71.7%); chloramphenicol, catI (85.1%); trimethoprim, dhfrI (76.3%); and sulfamethoxazole, sul1 (60%) and sul2 (63.3%). In the majority of cases, resistance to a given antimicrobial, except for streptomycin, was caused by a single gene. A negative association between tetA and tetB, between aac(3)II and aac(3)IV, and between dhfrI and dhfrV was observed. The present study gives baseline data on frequency and molecular basis of antimicrobial resistance in AEEC strains from ruminants.
机译：从小牛，羔羊和腹泻的山羊羔羊以及健康的牛，绵羊和山羊中分离出的226株附着和脱落的大肠杆菌（AEEC）菌株（20肠出血性大肠杆菌和206非典型肠致病性大肠杆菌）被分离出来。通过圆盘扩散法测试了它们对10种抗菌剂的抗性。通过聚合酶链反应分析抗性和中等菌株中主要抗性基因的存在。对四环素，链霉素，红霉素和磺胺甲恶唑的抗性菌株的总体百分比非常高（> 65％）。此外，还检测到了高水平的氨苄西林，氯霉素，甲氧苄啶和甲氧苄啶-磺胺甲恶唑的耐药性（约30％）。 AEEC菌株对庆大霉素和粘菌素非常敏感（> 90％）。由于反刍动物产生的AEEC可能导致人类疾病，因此当前研究中检测到的高频率抗菌素耐药性令人关注。对于每种抗菌剂，耐药菌株中的主要耐药基因为氨苄青霉素，bla（TEM）（97.1％）;四环素，tetA（76.7％）;庆大霉素，aac（3）II（80％）;链霉素，strA / strB（76.7％）和aadA（71.7％）;氯霉素，catI（85.1％）;甲氧苄啶，dhfrI（76.3％）;磺胺甲恶唑，sul1（60％）和sul2（63.3％）。在大多数情况下，除链霉素外，对给定抗菌素的耐药性是由单个基因引起的。观察到tetA和tetB之间，aac（3）II和aac（3）IV之间以及dhfrI和dhfrV之间存在负关联。本研究提供了反刍动物AEEC菌株中抗药性的频率和分子基础的基线数据。

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