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首页> 外文期刊>The Journal of Infectious Diseases >Atypical enteropathogenic Escherichia coli strains: phenotypic and genetic profiling reveals a strong association between enteroaggregative E. coli heat-stable enterotoxin and diarrhea.
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Atypical enteropathogenic Escherichia coli strains: phenotypic and genetic profiling reveals a strong association between enteroaggregative E. coli heat-stable enterotoxin and diarrhea.

机译：非典型肠致病性大肠埃希氏菌菌株：表型和基因分析揭示肠聚集性大肠杆菌热稳定肠毒素和腹泻之间有很强的联系。

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摘要

The virulence profiles of most atypical enteropathogenic Escherichia coli (EPEC) strains are unknown. A total of 118 typical and atypical strains of EPEC serotypes and non-EPEC serogroups isolated from children with or without acute diarrhea who were from different cities in Brazil were examined for virulence-associated markers and adherence to HEp-2 cells, and also had random amplified polymorphic DNA (RAPD) analysis performed. Atypical strains were identical to typical strains with regard to the virulence factors encoded on the locus of enterocyte effacement (LEE). In contrast with typical EPEC strains, none of the atypical strains reacted with the bfpA probe, and half of the strains hybridized with the perA probe. Most atypical strains presented Tir sequences that correlated with enteropathogenic or enterohemorrhagic E. coli (98%), had LEE inserted in either selC or pheU (88%), and presented a typeable intimin (52%). Eighteen new serotypes were found in the EPEC strains. Atypical and typical EPEC strains belonged to different RAPD clusters. Most atypical strains showed a localized-like adherence pattern (61.5%). Of the non-LEE-encoded virulence factors, enteroaggregative E. coli heat-stable enterotoxin was noted most frequently (45%) and was significantly associated with diarrhea (P=.01). Thus, this virulence marker may be used as an additional tool for the diagnosis of truly atypical pathogenic strains.
机译：大多数非典型肠致病性大肠埃希菌（EPEC）菌株的毒力特征尚不清楚。分别对来自巴西不同城市的118例典型和非典型EPEC血清型和非EPEC血清型菌株进行了分离，这些菌株分别来自巴西不同城市的有或没有急性腹泻的儿童，其毒力相关标志物和对HEp-2细胞的粘附性也随机进行扩增多态性DNA（RAPD）分析。就肠上皮细胞受损（LEE）基因座上编码的毒力因子而言，非典型菌株与典型菌株相同。与典型的EPEC菌株相比，非典型菌株均未与bfpA探针反应，一半菌株与perA探针杂交。大多数非典型菌株的Tir序列与肠致病性或肠出血性大肠杆菌相关（98％），LEE插入selC或pheU中（88％），并呈典型的内膜素（52％）。在EPEC菌株中发现了18种新的血清型。非典型和典型的EPEC菌株属于不同的RAPD簇。大多数非典型菌株表现出局部样的粘附模式（61.5％）。在非LEE编码的致病因子中，最常见的是肠聚合性大肠杆菌热稳定肠毒素（45％），并且与腹泻显着相关（P = .01）。因此，该毒力标记可以用作诊断真正非典型病原菌株的附加工具。

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