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首页> 外文期刊>Research Opinions in Animal & Veterinary Sciences >Genetic differentiation and gene flow between four Tunisian sheep populations ( Ovis aries ) using random amplified polymorphic DNA-polymerase chain reaction (RAPD-PCR) analysis
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Genetic differentiation and gene flow between four Tunisian sheep populations ( Ovis aries ) using random amplified polymorphic DNA-polymerase chain reaction (RAPD-PCR) analysis

机译：使用随机扩增多态性DNA-聚合酶链反应（RAPD-PCR）分析的四个突尼斯绵羊种群（Ovis aries）的遗传分化和基因流

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摘要

In this study, random amplified polymorphic DNA-polymerase chain reaction (RAPD-PCR) analysis was used to assess the genetic difference and gene flow among four sheep populations belonging to two native Tunisian breeds (the Barbarine and the Western thin tail). A total of 96 samples were typed using eight RAPD primers. 62 bands were scored, of which 44 bands (70.97%) were polymorphic. The unweighed pair-group method with arithmetic average (UPGMA) and principal component analysis (PCA) did not show a clear differentiation between populations within breeds. The coefficient of gene differentiation (Gst) between populations was relatively low. Analysis of molecular variance (AMOVA) showed that there was a genetic variation of 28.79% (F CT = 0.2879; P<0.001) among the breeds, 4.10% among populations within breeds (F SC = 0.0576; P<0.001)) and 67.10% (F ST = 0.3289; P<0.001) within populations. The level of gene migration between populations within breeds is quite high to homogenizing them.
机译：在这项研究中，使用随机扩增多态性DNA聚合酶链反应（RAPD-PCR）分析来评估属于两个突尼斯本地品种（Barbarine和Western细尾）的四个绵羊种群之间的遗传差异和基因流。使用八种RAPD引物对总共96个样品进行分型。得分为62条，其中44条（70.97％）是多态的。具有算术平均值（UPGMA）和主成分分析（PCA）的未加权成对组方法未显示出品种内种群之间的明显区别。种群之间的基因分化系数（Gst）相对较低。分子方差分析（AMOVA）显示，遗传变异在种之间为28.79％（F CT = 0.2879; P <0.001），在种内种群之间为4.10％（FSC = 0.0576; P <0.001））和67.10人群中的百分比（F ST = 0.3289; P <0.001）。使其同质化的种群之间的基因迁移水平很高。

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