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首页> 外文期刊>Research Opinions in Animal & Veterinary Sciences >Luteinizing hormone receptor and cytochrome P450scc enzyme mRNA expression within the gonad of mice injected with Serine/threonine or glycine
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Luteinizing hormone receptor and cytochrome P450scc enzyme mRNA expression within the gonad of mice injected with Serine/threonine or glycine

机译：丝氨酸/苏氨酸或甘氨酸注射后小鼠性腺中的黄体生成素受体和细胞色素P450scc酶mRNA表达

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摘要

The aim of this experiment was to test the effect of injecting serine/threonine or glycine on mRNA’s for Luteinizing Hormone Receptor (LHR) and cytochrome P450scc enzyme (P450ssc) expression in mouse testes and ovaries. Male and female mice (CD-1 strain) were injected with saline (S, control), serine/threonine (A, test) or glycine (G, test). Total RNA was extracted, transferred onto a nylon membrane and probed with 32P-labelled cDNA probes. The expression of mRNA’s for LHR and P450ssc were increased by serine/threonine and glycine injection in female while both of them were increased in male mice. The decrease in LHR and P450ssc mRNA expression, after serine/threonine or glycine injection, might be a result of changes in the function of PI3K or PKA enzymes and decreases in the phosphorylation of Sp1 and StAR proteins in the ovaries. The increases in LHR and P450ssc mRNA expression in testis indicating difference in regulatory mechanism between male and female.
机译：这项实验的目的是测试注射丝氨酸/苏氨酸或甘氨酸对小鼠睾丸和卵巢中的黄体生成激素受体（LHR）和细胞色素P450scc酶（P450ssc）表达的mRNA的影响。给雄性和雌性小鼠（CD-1品系）注射生理盐水（S，对照），丝氨酸/苏氨酸（A，测试）或甘氨酸（G，测试）。提取总RNA，转移到尼龙膜上，并用32P标记的cDNA探针探测。通过注射丝氨酸/苏氨酸和甘氨酸，雌性小鼠的LHR和P450ssc mRNA的表达增加，而雄性小鼠中的两者均升高。注射丝氨酸/苏氨酸或甘氨酸后，LHR和P450ssc mRNA表达的下降可能是PI3K或PKA酶功能改变以及卵巢Sp1和StAR蛋白磷酸化降低的结果。睾丸中LHR和P450ssc mRNA表达的增加表明男性和女性之间的调节机制存在差异。

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