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首页> 外文期刊>Research Opinions in Animal & Veterinary Sciences >Optimizing chemically-defined culture medium for the production of bovine ( Bos taurus ) embryos in vitro
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Optimizing chemically-defined culture medium for the production of bovine ( Bos taurus ) embryos in vitro

机译：优化化学成分确定的培养基以体外培养牛（Bos taurus）胚胎

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The study optimized and established an efficient protocol for in vitro production of bovine pre-implantation embryos. Specifically, it developed a protocol that will yield higher blastocyst rate and better embryo quality using the modified Synthetic Oviductal Fluid and modified Potassium Simplex Optimization Medium culture media. Aspirated and selected oocytes from slaughterhouse-derived ovaries were placed into an in vitro maturation medium for 22 h. In vitro fertilization was allowed for 16 to 18 h and presumptive zygotes were washed and placed on the six different treatment culture media containing different combinations of modified Synthetic Oviductal Fluid and modified Potassium Simplex Optimization Medium culture media and supplemented with bovine serum albumin and fetal calf serum. In sequential culture media, embryos were transferred after 3-4 days to the second treatment culture media. Cleavage rate and development to blastocyst was determined at 2 and 7 days after in vitro culture, respectively. Blastocysts were stained with Hoechst 33342-Propium Iodide differential staining to assess embryo quality. The rate of cleavage was not significantly different among all treatments; however, sequential culture of embryos in modified Synthetic Oviductal Fluid + 3mg/ml BSA for 1-3 days and Potassium Simplex Optimization Medium + 5% FCS for 4-7 days yielded higher blastocyst development and blastocyst cell number. Based on the results, it can be concluded that the use of modified Potassium Simplex Optimization Medium supplemented with fetal calf serum in the later part of in vitro culture is beneficial in bovine embryo development.
机译：这项研究优化并建立了体外植入牛植入前胚胎的有效方案。具体来说，它开发了一种方案，使用改良的合成输卵管液和改良的钾单纯形优化培养基可以产生更高的胚泡率和更好的胚胎质量。将来自屠宰场卵巢的抽吸卵母细胞和选择卵母细胞置于体外成熟培养基中22 h。允许体外受精16至18 h，将推定的受精卵洗涤并置于六种不同的处理培养基中，这些培养基中包含改良的合成输卵管液和改良的单纯钾优化培养基的不同组合，并补充了牛血清白蛋白和胎牛血清。在顺序培养基中，将胚胎在3-4天后转移到第二种处理培养基中。在体外培养后第2天和第7天分别测定卵裂率和向胚泡的发育。胚泡用Hoechst 33342-碘化丙啶差异染色法染色以评估胚胎质量。在所有治疗中卵裂率没有显着差异。但是，在改良的合成输卵管液+ 3mg / ml BSA中连续培养1-3天，在钾简单优化培养基+ 5％FCS中培养4-7天，可产生更高的胚泡发育和胚泡细胞数。根据这些结果，可以得出结论，在体外培养的后期使用改良的补充有小牛血清的钾简单优化培养基对牛胚胎发育是有益的。

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