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首页> 外文期刊>Research Opinions in Animal & Veterinary Sciences >Isolation of Coxiella burnetii from aborted fetuses of bovine and buffalo and its identification through nested and real-time PCR assays
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Isolation of Coxiella burnetii from aborted fetuses of bovine and buffalo and its identification through nested and real-time PCR assays

机译：从牛和水牛流产胎儿中分离出伯氏柯氏杆菌，并通过巢式和实时PCR分析鉴定

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摘要

The epidemiology and prevalence of Coxiella burnetii in aborted fetus of bovine and buffalo in various seasons and partums is unknown. This study was designed to find the prevalence of C. burnetii in bovine and buffalo in different areas, season, months and post-partum period through nested and real time polymerase chain reaction (PCR). Our results indicated that nested PCR 14 (4.68%) and 11 (4.29%) were positive for presence of C. burnetii. Real-time PCR showed that 19 (6.35%) and 13 (5.07%) of samples were positive for C. burnetii in aborted fetus of bovine and buffalo. Gilan (8.57%) had the highest and Boshehr (3.44%) Kerman and Ilam (0.0%) had the lowest prevalence of C. burnetii in aborted bovine and buffalo fetus. Our results indicated that bovine is more sensitive than buffalo to C. burnetii’s abortion (6.35% versus 5.07%). Our results showed that the prevalence of C. burnetii in winter (December-February), spring (March-May), summer (Jun-August) and autumn (September-November) of the year were 10.52%, 84.26%, 5.26% and 0.0% in cows and 23.07%, 69.23%, 0.0% and 7.69% in buffalo, respectively. From the total 19 bovine and 13 buffalos aborted fetuses, 14 (73.68%) and 8(61.53%) were positive from first partum respectively. No C. burnetii was positive in bovine and only 1 (7.69%) isolate was positive from buffalo in Fifth and last five partum.
机译：尚不清楚在不同季节和产期流产的牛和水牛胎儿的柯氏杆菌的流行病学和流行情况。本研究旨在通过巢式和实时聚合酶链反应（PCR）来发现牛，水牛在不同地区，季节，月份和产后不同时期在牛和水牛中的流行情况。我们的结果表明，巢式PCR 14（4.68％）和11（4.29％）呈阳性表达。实时PCR显示，在流产的牛和水牛胎儿中，伯氏梭菌呈阳性的样本为19（6.35％）和13（5.07％）。在流产的牛胎和水牛胎中，伯氏梭状芽胞杆菌的患病率最高，其中吉兰（8.57％）最高，波谢尔（3.44％）克尔曼和伊兰（0.0％）最低。我们的结果表明，牛比水牛对伯尼梭菌的流产更为敏感（6.35％对5.07％）。我们的结果表明，一年中冬季（12月至2月），春季（3月至5月），夏季（6月至8月）和秋季（9月至11月）的伯氏梭状芽胞杆菌患病率分别为10.52％，84.26％，5.26％奶牛分别为0.0％和0.0％，水牛分别为23.07％，69.23％，0.0％和7.69％。在总共19头牛和13头水牛流产的胎儿中，第一胎分别为14（73.68％）和8（61.53％）阳性。在第五胎和最后五个胎中，水牛没有伯氏梭状芽胞杆菌阳性，而水牛中只有1个（7.69％）分离株呈阳性。

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