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首页> 外文期刊>Revista de la Sociedad Espanola del Dolor >Método óptimo para la obtención de plasma rico en plaquetas en el Servicio de Clínica del Dolor del CMN 20 de noviembre ISSSTE
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Método óptimo para la obtención de plasma rico en plaquetas en el Servicio de Clínica del Dolor del CMN 20 de noviembre ISSSTE

机译:11月20日,ISSSTE,在CMN疼痛诊所获得获取富含血小板血浆的最佳方法

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Objective: To determine the optimal method to collect platelet-rich plasma (PRP). Material and methods: We collected 15 ml of blood from 36 healthy adults to determine platelet count and to test which of the following three protocols were the best to collect PRP. We used 3 ml of blood for each method: protocol 1: (n = 21) the blood tube was tested at 1200 revolutions per minute (RPM) in 2 centrifuge cycles of 8 minutes each; protocol 2 (n = 8): 1200 RPM/1 cycle/8 minutes, and protocol 3: (n = 7): 1200 RPM/1 cycle/10 minutes. From the blood samples that we collected, we also analyzed 1 ml of plasma to determine platelet performance by using an automatic flow cytometer (BD FACSCanto II). Platelet performance was determined by the following formula: platelet PRP count (100)/platelet count from total blood. The statistical method used were measures of central tendency, Chi-square, t-test, analysis of variance and the Wilcoxon and Kruskal-Wallis tests. Results: The basal overall count of platelet was 261.2 miles/mcl for the three methods (p = 0.906), 662.3 ± 243.3 (p = 0.001) for protocol 1; 377.9 ± 101.4 (p = 0.008) for protocol 2 and 30.9 ± 18.8 (p = 0.016) for protocol 3. Platelet performances were 255.2 ± 57.6 %; 149.3 ± 24.6 %; 13.3 ± 11.6 % respectively. Conclusion: Applying the first protocol we obtain PRP (1200 RPM/2 cycles of 8 minutes each). This study was done in healthy adults; however, future studies with a larger sample size are needed to confirm our findings of this method. In case of collecting platelets in studies with greater population being the adequate, its clinical utility should be validated by including patients with chronic and degenerative diseases and take care of associated diseases.
机译:目的:确定收集富血小板血浆(PRP)的最佳方法。材料和方法:我们从36位健康的成年人中采集了15毫升血液,以确定血小板计数并测试以下三种方案中哪种最适合采集PRP。我们为每种方法使用3毫升血液:方案1:(n = 21),在2个离心离心机循环中,分别以每分钟1200转(RPM)的速度测试血液,每次离心8分钟。协议2(n = 8):1200 RPM / 1个周期/ 8分钟,而协议3(n = 7):1200 RPM / 1周期/ 10分钟。从我们收集的血液样本中,我们还使用自动流式细胞仪(BD FACSCanto II)分析了1 ml血浆以确定血小板性能。血小板性能由下式确定:血小板PRP计数(100)/全血中的血小板计数。所使用的统计方法是集中趋势的度量,卡方检验,t检验,方差分析以及Wilcoxon和Kruskal-Wallis检验。结果:三种方法的血小板基础总计数为261.2英里/毫升(p = 0.906),方案1的血小板总计数为662.3±243.3(p = 0.001);方案2为377.9±101.4(p = 0.008),方案3为30.9±18.8(p = 0.016)。血小板性能为255.2±57.6%; 149.3±24.6%;分别为13.3±11.6%。结论:应用第一个协议可获得PRP(1200 RPM / 2个周期,每个周期8分钟)。这项研究是在健康成人中完成的;但是,未来需要进行更大样本量的研究以证实我们对这种方法的发现。如果在人群较多的研究中收集血小板足够,应通过纳入患有慢性和退行性疾病的患者并照顾相关疾病来验证其临床实用性。

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