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首页> 外文期刊>Revista Brasileira de Fruticultura >Temporary immersion biorreators: efficient technique for the propagation of the 'Pircinque' strawberry
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Temporary immersion biorreators: efficient technique for the propagation of the 'Pircinque' strawberry

机译：临时浸入式生物反应器：“ Pircinque”草莓繁殖的有效技术

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摘要

The in vitro propagation technique via temporary immersion bioreactors is a tool that, through the culture in a liquid medium, allows an increase in the efficiency of seedling production. Several researches with the strawberry crop have shown greater efficiency of the system compared to the conventional process of micropropagation in solid medium. In this sense, the objective herein was to establish a protocol of multiplication and rooting of the Pircinque strawberry, in temporary immersion bioreactors. Two distinct and independent studies were carried out, characterized by the multiplication and rooting stages of strawberry explants, newly introduced and registered in Brazil. Two culture media (MS and KNOP) were studied and, as a control treatment, the growth of the explants in solid culture medium was evaluated with the addition of 5 g L-1 of agar. Different immersion times of the culture medium were explored: five or eight times a day, for 15 minutes. The study was composed of the culture medium and immersion time factors, as well as the control (solid) treatment. It was verified that the use of temporary immersion bioreactors system is an efficient technique for the multiplication and rooting of explants of strawberry cv. Pircinque, when compared to the conventional method of micropropagation with the use of solid culture medium, making it possible to optimize the production of seedlings in biofactories. The MS liquid medium, in contact with explants of Pircinque strawberry five times a day, increased the growth of the aerial part and the root system.
机译：通过临时浸入式生物反应器进行的体外繁殖技术是一种工具，通过在液体培养基中进行培养，可以提高幼苗的生产效率。草莓作物的多项研究表明，与传统的在固体培养基中微繁殖的方法相比，该系统具有更高的效率。从这个意义上讲，本文的目的是建立在临时浸没式生物反应器中使Pircinque草莓繁殖和生根的方案。进行了两项截然不同的独立研究，其特征是在巴西新近引进和注册的草莓外植体的繁殖和生根阶段。研究了两种培养基（MS和KNOP），作为对照处理，通过添加5 g L-1琼脂来评估外植体在固体培养基中的生长。探索了培养基的不同浸入时间：每天五到八次，持续15分钟。该研究由培养基和浸入时间因素以及对照（固体）处理组成。事实证明，使用临时浸没式生物反应器系统是草莓cv外植体繁殖和生根的有效技术。与使用固体培养基的传统微繁殖方法相比，Pircinque可以优化生物工厂中幼苗的生产。 MS液体培养基每天与Pircinque草莓的外植体接触五次，可增加地上部分和根系的生长。

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