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首页> 外文期刊>Revista Brasileira de Fruticultura >Embriogênese somática do caquizeiro
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Embriogênese somática do caquizeiro

机译：柿的体细胞胚发生

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The goal of this work was to develop a protocol for persimmon cloning through somatic embryogenesis. Zygotic embryos excised from fruits collected from adult plants were tested at several developmental phases. They were collected during 22 weeks after 4 weeks blossoming. The basic medium tested was MS?NO3. Initial induction medium was supplemented with 20 μM 2,4-D + 2 μM kinetin. Dark calluses obtained were transferred to induction medium, with concentrations of 10 and 20 μM 2,4-D + 2 μM kinetin. The calli with pro-embryogenic masses were transferred to a maintenance and multiplication medium, with 2 μM kinetin and 2,4-D at the concentrations of 2.5, 5.0 and 10 μM. Embryogenic masses formed were transferred to a maturation medium supplemented with 0.5 μM IBA and 5, 10 and 20 μM 2iP. Embryos formed were isolated in two conversion media, one with 5 μM 2-iP + 5 μM GA3 and 0.5 μM IBA, and another medium with 0.5 μM GA3 and BAP at 0, 0.25, 0.5 and 1.0 μM. Indirect somatic embryogenesis were obtained from mature zygotic embryos collected after 22 weeks, when cultivated in culture medium with 10 μM 2,4D combined with 2 μM kinetin. Embryos maintenance and multiplication were more efficient with 5 μM 2,4-D, in which the pro-embryos advanced to the globular embryos phase. At the maturation phase the concentrations of 2-iP tested promoted globular embryos to more advanced stages of ontogeny, such as cordiform, torpedo and cotiledonary. Supplementation of the culture medium with 1 μM BAP generated better developed plants, with highest number of leaves and size.
机译：这项工作的目的是开发通过体细胞胚发生克隆柿子的方案。从成年植物收集的果实中切下的合子胚在几个发育阶段进行了测试。在开花4周后的22周内收集它们。测试的基本培养基为MS？NO3。初始诱导培养基中添加了20μM2,4-D + 2μM激动素。将获得的深色老茧转移至浓度为10和20μM2,4-D + 2μM激动素的诱导培养基中。将具有促胚性肿块的愈伤组织转移到维持和繁殖培养基中，该培养基中含有浓度分别为2.5、5.0和10μM的2μM激动素和2,4-D。将形成的胚发生团转移到补充有0.5μMIBA和5、10和20μM2iP的成熟培养基中。在两种转化培养基中分离出形成的胚，一种具有5μM2-iP + 5μMGA3和0.5μMIBA，另一种具有0.5μMGA3和BAP分别在0、0.25、0.5和1.0μM的培养基。当在含有10μM2,4D和2μM激动素的培养基中培养时，从22周后收集的成熟合子胚胎获得间接体细胞胚发生。胚胎的维持和繁殖在5μM2,4-D条件下更为有效，其中前胚已发展到球状胚期。在成熟阶段，所测试的2-iP浓度可将球状胚胎提升至个体发育的更高级阶段，例如堇形，鱼雷和齿骨。补充1μMBAP的培养基可产生更好的植株，并具有最多的叶片数和最大的大小。

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