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首页> 外文期刊>Revista Brasileira de Fruticultura >Indu??o de calo a partir de eixo embrionário de coqueiro (Cocos nucifera L.)
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Indu??o de calo a partir de eixo embrionário de coqueiro (Cocos nucifera L.)

机译：椰子树（Cocos nucifera L.）胚轴的愈伤组织诱导

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The ability of callus formation from the embryo axes of coconut in different concentrations of 2,4-D was evaluated. The experimental design was completely randomized in a 4x5 factorial scheme (4 concentrations of 2,4-D x 5 segments of the embryogenic axis). The axes were excised longitudinally out of zigotic embryos and were then disinfected with sodium hipocloride (0.2%) for two minutes, washed with sterile distilled water, and soaked for two minutes in a solution of sterile citric acid (100 mg.L-1). The embryogenic axes were split in five segments corresponding to positions A, B, C, D and E, and transferred to Petri dishes containing the culture media Y3, suplemented with four concentrations of 2,4-D (10-4; 1.36x10-4; 3.62x10-4; 4.52x10-4 M), sucrose (50 g.L-1), activated charcoal (2.5 g.L-1) and vitamins of Morel and Wetmore. The Petri dishes were incubated in a dark atmosphere under the temperature of 25 ± 2oC. After 15 days of inoculation, the segments A and B in the concentration of 10-4 M of 2,4-D presented 97.5% of explants with friable callus, and 92,5% and 80%, respectively, in the concentrations of 1.36x10-4 M of 2,4-D. The E segment, in both concentrations, presented 60% of callus formation. After 30 days of inoculation, the segments A and B promoted 100% and 97.5% in the concentration of 10-4 M, and 90% and 80%, respectively, in the concentrations of 1.36x10-4 M. The E segment promoted 55% to 57.5% of callus induction in both concentrations.
机译：评价了椰子在不同浓度的2，4-D中从胚轴形成愈伤组织的能力。实验设计以4x5因子分解方案（4种浓度的2，4-D x 5个胚发生轴片段）完全随机分配。将轴从锯齿状胚胎中纵向切除，然后用盐酸希波利特（0.2％）消毒2分钟，用无菌蒸馏水洗涤，然后在无菌柠檬酸（100 mg.L-1）溶液中浸泡2分钟。 。将胚胎发生轴分为对应于位置A，B，C，D和E的五个部分，并转移到含有培养基Y3的陪替氏培养皿中，并添加四种浓度的2,4-D（10-4; 1.36x10- 4; 3.62x10-4; 4.52x10-4 M），蔗糖（50 gL-1），活性炭（2.5 gL-1）和羊肚菌和湿肉的维生素。培养皿在25±2℃的黑暗气氛中孵育。接种15天后，浓度为10-4 M的2,4-D的A区和B区呈现出97.5％的带有脆性愈伤组织的外植体，分别占浓度为1.36的92.5％和80％。 x10-4 M为2,4-D。两种浓度下的E区段均占愈伤组织形成的60％。接种30天后，片段A和B在10-4 M的浓度下分别促进100％和97.5％，在1.36x10-4 M的浓度下分别促进90％和80％。E片段在55时促进55两种浓度下愈伤组织诱导的％至57.5％。

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