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首页> 外文期刊>Revista Cubana de Hematología, Inmunología y Hemoterapia >Frecuencia del HLA-B*27:: algunas consideraciones sobre su uso como herramienta diagn?3stica
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Frecuencia del HLA-B*27:: algunas consideraciones sobre su uso como herramienta diagn?3stica

机译：HLA-B的频率* 27：有关将其用作诊断工具的一些注意事项

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The detection by molecular methods of the HLA-B*27 allele has become in recent years in Cuba in a tool that supports the diagnosis of some diseases generally known as spondyloarthropathies (SpA).In order to determine the percentage of the gene positivity in individuals with clinical suspicion of SpA and uveitis, we studied 236 DNA samples characterized in gender, age and skin color, of patients with HLA-B*27 indication.The genotyping was performed by Real Time - PCR with the LightMix HLA-B*27 SimpleProbe kit (TIBMOLBIOL). The 42.7% of the cases were older than 40 years, with a mean of 39 years old and a male - female ratio of 0.84; however, patients debuting with HLA-B*27-related diseases should be predominantly males between the second and third decade of life. Our results showed that 188 (78.4%) were negative and only 51 (21.6%) were positive however, according to the type of pathology, the frequency of this gene should be between 36.0% and 90.0%.The statistical differences were only associate to gender but not in the groups of different skin color and presumptive diagnosis. The 18.0% of the laboratory test request forms had no diagnostic impression and these cases had the lowest positivity rates. The present results show that the requests for HLA-B*27 generated in the Cuban health system do not follow uniform criteria of clinical suspicion of SpA and uveitis; and that request forms do not include the necessary data to allow a correct interpretation of this immunogenetic test.
机译：近年来，通过分子方法检测HLA-B * 27等位基因已成为古巴使用的一种工具，该工具可支持某些通常被称为脊椎关节病（SpA）的疾病的诊断。为了确定个体中基因阳性的百分比由于临床上怀疑有SpA和葡萄膜炎，我们研究了236例HLA-B * 27适应症患者的性别，年龄和肤色特征的DNA样本。通过实时荧光定量PCR技术对LightMix HLA-B * 27 SimpleProbe进行基因分型套件（TIBMOLBIOL）。 42.7％的病例年龄大于40岁，平均39岁，男女之比为0.84;但是，首次出现HLA-B * 27相关疾病的患者在生命的第二个到第三个十年之间应该主要是男性。我们的结果显示188（78.4％）为阴性，只有51（21.6％）为阳性，但根据病理类型，该基因的频率应在36.0％至90.0％之间。统计学差异仅与性别，但不能区分肤色和推定诊断。 18.0％的实验室测试申请表没有诊断印象，这些病例的阳性率最低。目前的结果表明，古巴卫生系统对HLA-B * 27的要求并未遵循临床上对SpA和葡萄膜炎的怀疑的统一标准。并且该申请表未包含必要的数据，无法正确解释该免疫原性测试。

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