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首页> 外文期刊>Reviews of Reproduction >Involvement of nephrin in human placental trophoblast syncytialization
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Involvement of nephrin in human placental trophoblast syncytialization

机译：肾素参与人胎盘滋养细胞合胞体化

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The placenta has numerous functions, such as transporting oxygen and nutrients and building the immune tolerance of the fetus. Cell fusion is an essential process for placental development and maturation. In human placental development, mononucleated cytotrophoblast (CTB) cells can fuse to form a multinucleated syncytiotrophoblast (STB), which is the outermost layer of the placenta. Nephrin is a transmembrane protein that belongs to the Ig superfamily. Previous studies have shown that nephrin contributes to the fusion of myoblasts into myotubes in zebrafish and mice, presenting a functional conservation with its Drosophila ortholog sticks and stones. However, whether nephrin is involved in trophoblast syncytialization remains unclear. In this study, we report that nephrin was localized predominantly in the CTB cells and STB of human placenta villi from first trimester to term pregnancy. Using a spontaneous fusion model of primary CTB cells, the expression of nephrin was found to be increased during trophoblast cell fusion. Moreover, the spontaneous syncytialization and the expression of syncytin 2, connexin 43, and human chorionic gonadotropin beta were significantly inhibited by nephrin-specific siRNAs. The above results demonstrate that nephrin plays an important role in trophoblast syncytialization.
机译：胎盘具有多种功能，例如运输氧气和营养物质以及增强胎儿的免疫耐受性。细胞融合是胎盘发育和成熟的重要过程。在人类胎盘发育中，单核细胞滋养层（CTB）细胞可以融合形成多核合体滋养层（STB），这是胎盘的最外层。肾素是属于Ig超家族的跨膜蛋白。先前的研究表明，nephrin有助于成肌细胞融合到斑马鱼和小鼠的肌管中，并通过果蝇直系同源基因的果蝇棒和石头表现出功能保守性。然而，尚不清楚肾素是否参与滋养细胞的合胞体化。在这项研究中，我们报道从早孕到足月妊娠，nephrin主要位于人胎盘绒毛的CTB细胞和STB中。使用原代CTB细胞的自发融合模型，发现nephrin的表达在滋养层细胞融合过程中增加。此外，肾素特异性siRNA可以显着抑制自发合胞作用以及合胞素2，连接蛋白43和绒毛膜促性腺激素β的表达。上述结果表明，肾素在滋养细胞合胞体化中起重要作用。

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