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Differences in the metabolomic signatures of porcine follicular fluid collected from environments associated with good and poor oocyte quality

机译：从与卵母细胞质量好坏有关的环境中收集的猪卵泡液代谢组学特征的差异

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The microenvironment of the developing follicle is critical to the acquisition of oocyte developmental competence, which is influenced by several factors including follicle size and season. The aim of this study was to characterise the metabolomic signatures of porcine follicular fluid (FF) collected from good and poor follicular environments, using high-resolution proton nuclear magnetic resonance (1H-NMR) spectroscopy. Sow ovaries were collected at slaughter, 4 days after weaning, in summer and winter. The contents of small (3–4?mm) and large (5–8?mm) diameter follicles were aspirated and pooled separately for each ovary pair. Groups classified as summer-small (n=8), summer-large (n=15), winter-small (n=9) and winter-large (n=15) were analysed by 1H-NMR spectroscopy. The concentrations of 11 metabolites differed due to follicle size alone (P<0.05), including glucose, lactate, hypoxanthine and five amino acids. The concentrations of all these metabolites, except for glucose, were lower in large FF compared with small FF. Significant interaction effects of follicle size and season were found for the concentrations of glutamate, glycine, N-acetyl groups and uridine. Succinate was the only metabolite that differed in concentration due to season alone (P<0.05). The FF levels of progesterone, androstenedione and oestradiol were correlated with the concentrations of most of the metabolites examined. The results indicate that there is a distinct shift in follicular glucose metabolism as follicles increase in diameter and suggest that follicular cells may be more vulnerable to oxidative stress during the summer months. Our findings demonstrate the power of 1H-NMR spectroscopy to expand our understanding of the dynamic and complex microenvironment of the developing follicle.
机译：卵泡的微环境对卵母细胞发育能力的获得至关重要，卵泡的微环境受卵泡大小和季节等几个因素的影响。这项研究的目的是使用高分辨率质子核磁共振波谱（1H-NMR）表征从良好和不良卵泡环境收集的猪卵泡液（FF）的代谢组学特征。在夏季和冬季，断奶后4天在屠宰时收集母猪卵巢。分别吸取小直径（3–4？mm）和大直径（5–8？mm）的卵泡，并分别合并每个卵巢。通过1 H-NMR光谱分析分为夏季小（n = 8），夏季大（n = 15），冬季小（n = 9）和冬季大（n = 15）的组。 11种代谢物的浓度仅因卵泡大小而异（P <0.05），包括葡萄糖，乳酸，次黄嘌呤和5种氨基酸。与小FF相比，大FF中所有这些代谢物的浓度（葡萄糖除外）均较低。谷氨酸，甘氨酸，N-乙酰基和尿苷的浓度对卵泡大小和季节的相互作用具有显着影响。琥珀酸盐是唯一因季节不同而浓度不同的代谢产物（P <0.05）。孕酮，雄烯二酮和雌二醇的FF水平与所检查的大多数代谢物的浓度相关。结果表明，随着卵泡直径的增加，卵泡葡萄糖代谢发生明显变化，这表明在夏季，卵泡细胞可能更容易受到氧化应激的影响。我们的发现证明了1H-NMR光谱的功能可扩展我们对发育中卵泡动态和复杂微环境的理解。

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