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首页> 外文期刊>Revista Brasileira de Fruticultura >Estabelecimento e multiplica??o in vitro de porta-enxertos de Prunus
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Estabelecimento e multiplica??o in vitro de porta-enxertos de Prunus

机译：李属砧木的建立及体外繁殖

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摘要

The success of the modern fruit production depend on the genetic and sanitary quality of plants. For the peach tree culture, micropropagation techniques allow a mass clonal production with high genetic quality plants. The present study aimed to evaluate the explant survival rate during in vitro establishment period, as well as to evaluate the in vitro multiplication potential of Prunus rootstocks. Apexes and lateral buds of the rootstocks Capdeboscq and GF677 and of the selection VP411 were introduced in vitro in the Lepoivre's medium supplemented with BAP (0.5mg.L-1). The mean values of explant survival for the rootstocks were 62.9% and 58.8%, for apexes and lateral buds, respectively. Cultures of apexes and lateral buds showed 14.8% and 29.8% of contamination, respectively. The genotype significantly affected the in vitro multiplication rates. The rootstocks Capdeboscq and GF677, and the selection VP411 showed multiplication rates of 14.7, 10.5 and 16.0 shoots/explant, respectively. As mean values for shoot height of the rootstocks Capdeboscq and GF677 and of the selection VP411, it was observed 9.3, 8.9 and 7.8 mm, respectively. The rootstock Capdeboscq showed superior values as regarded to the number of shoots >20mm as compared to rootstock GF677 and selection VP411 with values of 2.0, 1.3 and 1.0 of shoots, respectively.
机译：现代水果生产的成功取决于植物的遗传和卫生质量。对于桃树文化，微繁殖技术可以利用高遗传质量的植物进行大规模克隆生产。本研究旨在评估在体外建立期间外植体的存活率，以及评估李子砧木的体外繁殖潜力。在补充了BAP（0.5mg.L-1）的Lepoivre培养基中体外引入了砧木Capdeboscq和GF677以及选择VP411的根尖和侧芽。根茎和侧芽的砧木的外植体存活平均值分别为62.9％和58.8％。顶芽和侧芽的培养物分别显示出14.8％和29.8％的污染。基因型显着影响体外繁殖率。砧木Capdeboscq和GF677以及选择VP411的繁殖率分别为14.7、10.5和16.0枝/植株。作为砧木Capdeboscq和GF677以及选择VP411的苗高的平均值，分别观察到9.3、8.9和7.8毫米。相对于砧木GF677和选择VP411，砧木Capdeboscq的芽数> 20mm表现出更高的值，分别为2.0、1.3和1.0。

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