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Sequencing and de novo assembly of bacterial genomes: an approach to study new

机译:细菌基因组的测序和从头组装:研究新细菌的方法

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Next Generation Sequencing technologies have revolutionized the field of genomics allowing sequencing of a large number of genomes in a short period of time. The genome provides, in principle, the complete catalogue of genes which can be expressed by a microorganism. But the interpretation of this information, at each level (from the huge volume of raw data originated by the sequencer to the thousands of genes identified in parallel associated with various functions), is a major challenge from the computational point of view. De novo assembly is one of the approaches used to reconstruct genomes, particularly bacterial genomes. In this paper the main trends regarding sequencing, and specifically the de novo assembly, are reviewed.
机译:下一代测序技术彻底改变了基因组学领域,允许在短时间内对大量基因组进行测序。基因组原则上提供了可以由微生物表达的基因的完整目录。但是,从计算的角度来看,在每个级别(从由测序仪产生的大量原始数据到与各种功能并行关联的数千个基因)的这种信息的解释都是一个重大挑战。从头组装是用于重建基因组,尤其是细菌基因组的方法之一。在本文中,对测序的主要趋势,特别是从头组装进行了回顾。

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