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首页> 外文期刊>Revista de salud animal >A POLYCLONAL -ANTIBODY-IMMUNOPEROXIDASE-CONJUGATE FOR THE SPECIFIC DETECTION OF PORCINE CIRCOVIRUS TYPE 2
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A POLYCLONAL -ANTIBODY-IMMUNOPEROXIDASE-CONJUGATE FOR THE SPECIFIC DETECTION OF PORCINE CIRCOVIRUS TYPE 2

机译：多克隆抗体-免疫-过氧化物酶-共轭物，用于特异性检测2型猪圆环病毒

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摘要

Porcine circovirus 2 is nowadays accepted as the essential infectious agent of postweaning multisystemic wasting syndrome which causes severe economic losses in porcine production worldwide. The diagnosis of PMWS is a difficult task and must follow three criteria: (i) the presence of compatible clinical signs, (ii) the presence of characteristic microscopic histopathological lesions, and (iii) the presence of PCV2 within these lesions in moderate or high. The presence of PCV2 in lymphoid tissues must be demonstrated by in situ hybridization or immunohistochemical methods. The in situ hybridization is a more complex and expense compared to other diagnostic tools, on the other hand, one problem concerning the immunohistochemical methods for PMWS diagnostic is the lack of a commercial anti-PCV2 peroxidase conjugate; therefore, the aim of this work was to obtain a polyclonal-antibody-immunoperoxidase-conjugate for the PCV2 specific detection. An anti-PCV2-peroxidase conjugated for the PCV2 specific detection was obtained based on the use of the available commercial vaccine against PCV2 as immunogenic inoculation for producing a polyclonal antibody in rabbits. The conjugate obtained was able to discriminate between PCV2 and PCV1 infections and a high sensitivity and specificity of the conjugate were observed.
机译：如今，猪圆环病毒2被认为是断奶后多系统消耗综合症的重要传染原，该综合症在全世界的猪肉生产中造成严重的经济损失。 PMWS的诊断是一项艰巨的任务，必须遵循三个标准：（i）是否存在相适应的临床体征;（ii）是否存在特征性微观组织病理学病变;（iii）中度或高度病变的这些病变中是否存在PCV2 。必须通过原位杂交或免疫组织化学方法证明淋巴组织中PCV2的存在。与其他诊断工具相比，原位杂交更复杂，费用也更高。另一方面，有关用于PMWS诊断的免疫组化方法的一个问题是缺少商业化的抗PCV2过氧化物酶偶联物。因此，这项工作的目的是获得用于PCV2特异性检测的多克隆抗体-免疫过氧化物酶-缀合物。基于可利用的针对PCV2的商业疫苗作为免疫原性接种以在兔中产生多克隆抗体的使用，获得了用于PCV2特异性检测的抗PCV2-过氧化物酶。获得的结合物能够区分PCV2和PCV1感染，并且观察到结合物的高灵敏度和特异性。

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