Objective. To evaluate the effect of cholesterol and dimethyl formamide on the cryosurvival of colombian creole stallion sperm. Materials and methods. Semen from ten stallions was collected and frozen using the same protocol, with variations in the cryoprotectant (glycerol 5% or 5% DMF) and the addition or absence of cholesterol as membrane modifier (1.5 mg cholesterol loaded cyclodextrins for each 120 x 10Z6 cells). The cryosurvival was evaluated by estimating motility using the SCA software. The vitality and membrane integrity was estimated using eosin nigrosine staining and by hypo-osmotic test respectively. Results. Sperm incubated with cholesterol had a significant effect in improving the percentage of total motility, progressive motility, sperm velocity and percentage of rapid sperm along with a decreased percentage of sperm with slow motility. Additionally, there was an improvement in the percentage of live spermatozoa and spermatozoa with membrane integrity (p0.05). Dimethyl formamide (DMF) as a cryoprotectant, improved all the evaluated parameters in comparison with glycerol (p0.05). Conclusions. Both procedures improved the evaluated parameters due to the additive effects of the cryoprotectant and the membrane modifier, but there was no interaction between these two factors.
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机译：目的。评估胆固醇和二甲基甲酰胺对哥伦比亚克里奥尔种马精子冷冻存活的影响。材料和方法。收集来自十种种马的精液，并使用相同的方法冷冻，其中有不同的冷冻保护剂（5％甘油或5％DMF甘油）以及添加或不存在作为膜修饰剂的胆固醇（每120 x 10Z6细胞含1.5 mg胆固醇的环糊精） 。通过使用SCA软件评估运动能力来评估冷冻存活率。活力和膜完整性分别用曙红黑质己内酰胺染色和低渗试验评估。结果。与胆固醇一起孵育的精子在提高总运动性百分比，进行性运动性，精子速度和快速精子百分比以及降低慢运动性精子百分比方面具有显著作用。此外，活精子和具有膜完整性的精子的百分比有所提高（p <0.05）。与甘油相比，作为防冻剂的二甲基甲酰胺（DMF）改善了所有评估参数（p <0.05）。结论。由于冷冻保护剂和膜改性剂的累加作用，两种方法均改善了评估的参数，但是这两个因素之间没有相互作用。
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