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首页> 外文期刊>Revista MVZ Cordoba >Detection of equine herpesvirus 1 and 4 and its association with latency-associated transcripts in naturally infected horses in Colombia
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Detection of equine herpesvirus 1 and 4 and its association with latency-associated transcripts in naturally infected horses in Colombia

机译：哥伦比亚自然感染马匹中马疱疹病毒1和4的检测及其与潜伏期相关转录本的关联

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Objective. Determine the presence of antibodies and viral genomes of EHV-1 and EHV-4, as well as to detect the presence of latency associated transcripts (LATs) in a selected population of Colombian horses. Materials and methods. Serum samples, submandibular lymph nodes and trigeminal ganglion were obtained from 50 horses and analyzed. Sera were evaluated for the presence of antibodies against EHV-1 and EHV-4 while tissues were initially evaluated for the presence of viral genome by nPCR. Finally, samples were used for the detection of LATs through RT-PCR. Results. In general, 6/50 samples showed antibodies to EHV-1 and 44/50 were positive for EHV-4. As for viral genome detection, 10/50 samples were positive for EHV-1 and 30/50 were positive for EHV-4; in addition, 22/35 horses positive for EHV DNA were positive for LATs. The use of these tests led to eight possible combinations of results. Conclusions. The evidence used shows that horses can have simple viral infection, co-infections with both viruses, latency due to the presence of LATs and the simultaneous presence of LATs and viral genome replication at a given time. It contributes to the understanding of the behavior of the disease in Colombia and calls attention to the importance of implementing complementary diagnoses to the serology for the control of these viruses.
机译：目的。确定EHV-1和EHV-4的抗体和病毒基因组的存在，以及检测哥伦比亚马群中潜伏期相关的转录本（LAT）的存在。材料和方法。从50匹马中获取血清样本，下颌下淋巴结和三叉神经节并进行分析。评估血清中是否存在针对EHV-1和EHV-4的抗体，而最初通过nPCR评估组织中病毒基因组的存在。最后，将样品用于通过RT-PCR检测LAT。结果。一般而言，6/50样品显示抗EHV-1的抗体，而44/50则显示EHV-4呈阳性。至于病毒基因组检测，EHV-1阳性的样本为10/50，EHV-4阳性的样本为30/50;此外，EHV DNA阳性的22/35匹马的LAT阳性。这些测试的使用导致结果的八种可能的组合。结论。所使用的证据表明，在给定的时间，马可能具有简单的病毒感染，两种病毒的共同感染，由于存在LAT以及同时存在LAT和病毒基因组复制而导致的潜伏期。它有助于人们了解哥伦比亚的疾病行为，并引起人们对针对血清学进行补充诊断以控制这些病毒的重要性的关注。

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