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首页> 外文期刊>Romanian Journal of Biophysics >A SIMPLE ACCURATE MULTI-COMPONENT SPECTROPHOTOMETRIC METHOD FOR SIMULTANEOUS DETERMINATION OF TOTAL HEMOGLOBIN AND FOUR CLINICALLY SIGNIFICANT HEMOGLOBIN DERIVATIVES IN HUMAN AND RAT BLOOD
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A SIMPLE ACCURATE MULTI-COMPONENT SPECTROPHOTOMETRIC METHOD FOR SIMULTANEOUS DETERMINATION OF TOTAL HEMOGLOBIN AND FOUR CLINICALLY SIGNIFICANT HEMOGLOBIN DERIVATIVES IN HUMAN AND RAT BLOOD

机译：同时测定人血和大鼠血中总血红蛋白和四种临床上重要的血红蛋白衍生物的简便准确的多组分分光光度法

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Our recent multi-component spectrophotometric method for determination of hemoglobin derivatives has been developed theoretically and experimentally to get more accurate and precise concentrations of sulfhemoglobin, methemoglobin, carboxyhemoglobin and oxyhemoglobin in the blood of humans and rats. Theoretically, the equations used for determining the hemoglobin derivatives were derived by using more recent millimolar absorptivity matrices and are based on the theory of multi-component spectrophotometric analysis and the mathematical Gaussian elimination method for matrix calculation. New millimolar absorptivities at λ = 576 and 630 nm have been usedfor the first time to derive these equations. Experimentally, the method has been standardized with respect to the pH and temperature of hemoglobin solution. The results on methemoglobin percentage showed high correlations ( r = 0.997 and r = 0.998) with our old multi-component method and the chemical method, respectively. Also, the results of oxyhemoglobin determined by our new method showed a high correlation ( r = 0.999) with those determined by our old multi-component method. Moreover, the results of total hemoglobin concentration determined by our new method showed a high correlation ( r = 0.998) with the chemical method. These results indicate the high accuracy of our new method. Furthermore, the percent values of sulfhemoglobin, carboxyhemoglobin and oxyhemoglobin showed a coefficient of variation of 1.52%, 5.85% and 0.137%, indicating the high reproducibility and precision of our new method. The method is non-expensive, highly sensitive, accurate, precise and reproducible and have the advantages of small sample volume, simplicity and speed and can be computerized.
机译：从理论上和实验上，我们最近开发了用于测定血红蛋白衍生物的多组分分光光度法，以在人和大鼠的血液中获得更准确准确的硫代血红蛋白，高铁血红蛋白，羧基血红蛋白和氧合血红蛋白浓度。从理论上讲，用于确定血红蛋白衍生物的方程式是使用较新的毫摩尔吸收率矩阵推导的，并基于多组分分光光度分析理论和数学高斯消去法进行矩阵计算。首次使用了在λ= 576和630 nm处的新毫摩尔吸收率来推导这些方程。实验上，该方法已针对血红蛋白溶液的pH和温度进行了标准化。高铁血红蛋白百分比的结果分别与我们的旧的多组分方法和化学方法具有高度相关性（r = 0.997和r = 0.998）。同样，通过我们的新方法测定的氧合血红蛋白的结果与通过我们的旧多组分方法测定的结果显示出高度相关性（r = 0.999）。而且，通过我们的新方法测定的总血红蛋白浓度的结果与化学方法显示出高度相关性（r = 0.998）。这些结果表明我们的新方法具有很高的准确性。此外，硫血红蛋白，羧基血红蛋白和氧合血红蛋白的百分数显示出1.52％，5.85％和0.137％的变异系数，表明我们的新方法具有很高的重现性和精密度。该方法是廉价的，高度灵敏的，准确的，精确的和可重复的，并且具有样品体积小，简单和快速的优点并且可以被计算机化。

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