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STIMULATION AND CONTROL OF E. COLI BY USING EXTREMELY LOW FREQUENCY MAGNETIC FIELD

机译:极低频磁场对大肠杆菌的刺激与控制

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The effect of a 50 Hz magnetic field of strength 2 mT on each of the growth characteristics, the antibiotic sensitivity and the ultra structure of E. coli bacteria cells have been studied. Equal volumes of E. coli cells were exposed to the magnetic field for different periods, the two most effective periods, namely, 6 h and 16 h were chosen for all our experimental studies. The results indicated that exposure of the microorganisms to the demonstrated magnetic field caused pronounced changes in the growth characteristic curves, where a suppressive effect was observed on the cell growth and the number of cells at stationary phase markedly decreased after exposure period of 6 h but there was a slight increase in the growth rate after exposure period of 16 h with increase in the number of cells. Further, changes in the antibiotic sensitivity was observed after exposure period of 6 h since E. coli cells became more sensitive to certain antibiotics such as amoxicillin, nalidixic acid and erythromycin as revealed in the increase in their zone diameters while, after a 16 h exposure period, it became more resistant to the same antibiotics. Furthermore, the results of the ultra structure showed that while exposure period 6 h decreased the cell length, the exposure period 16 h elongated the cell length with decreasing the thickness of the cell wall beside the disappearance of the majority of cytoplasmic components.
机译:已经研究了强度为2 mT的50 Hz磁场对大肠杆菌细胞的每种生长特性,抗生素敏感性和超微结构的影响。等体积的大肠杆菌细胞在磁场中暴露的时间不同,我们所有的实验研究都选择了两个最有效的时间,即6小时和16小时。结果表明,将微生物暴露于已证实的磁场会导致生长特性曲线发生明显变化,其中对细胞生长具有抑制作用,并且在暴露6 h后,固定相的细胞数量明显减少,但存在暴露16 h后,随着细胞数量的增加,生长速率略有增加。此外,在暴露6小时后观察到抗生素敏感性的变化,因为大肠杆菌细胞对某些抗生素(如阿莫西林,萘啶酸和红霉素)变得更敏感,这表现为它们的区域直径增加,而暴露16 h后在此期间,它对相同的抗生素更具抵抗力。此外,超微结构的结果表明,虽然暴露时间6h减少了细胞长度,但暴露时间16h延长了细胞长度,同时细胞壁的厚度减小了,同时大多数细胞质成分消失了。

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