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Extremely low frequency magnetic field exposure affects DnaK and GroEL expression in E. coli cells with impaired heat shock response

机译:极低频率的磁场暴露会影响热休克反应的大肠杆菌细胞中DnaK和GroEL表达

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In our earlier experiments, we found that extremely low frequency magnetic fields (ELF-MF) affect heat shock protein (HSP) expression in wild type Escherichia coli cells. In the present work we investigate the ability of ELF-MF exposure to trigger an increase of DnaK and GroEL protein levels also in E. coli cells not exhibiting the classic heat shock response (HSR) when subjected to a 42°C heat stress. We find that these cells, although lacking a HSR to heat shock treatment, show an enhancement of DnaK and GroEL protein levels after 30 or 90 min sinusoidal ELF-MF exposure (50 Hz, 1 mT). This result suggests that the HSP induction pathway triggered by ELF-MF exposure could be different from that elicited by heat shock treatment.
机译:在我们较早的实验中,我们发现极低频磁场(ELF-MF)影响野生型大肠杆菌细胞中热激蛋白(HSP)的表达。在当前的工作中,我们研究了暴露于ELF-MF的能力也导致在承受42°C热应激时不表现出经典热休克反应(HSR)的大肠杆菌细胞中DnaK和GroEL蛋白水平升高。我们发现,这些细胞尽管缺乏热休克的热休克处理,但在正弦曲线的ELF-MF暴露(50 Hz,1 mT)30或90分钟后显示出DnaK和GroEL蛋白水平的增强。该结果表明,由ELF-MF暴露触发的HSP诱导途径可能不同于热休克治疗所诱导的途径。

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