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首页> 外文期刊>Romanian Journal of Laboratory Medicine >The role of the sialic acid in monitoring the evolution of malignant melanoma. From murine models to human research
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The role of the sialic acid in monitoring the evolution of malignant melanoma. From murine models to human research

机译：唾液酸在监测恶性黑色素瘤演变中的作用。从鼠模型到人类研究

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Purpose: To identify sialic acid as a tumor marker to be used in experimental models. Obtained data will be extrapolated to humans, so that this marker can be used in clinical practice. Materials and methods: We used B16 melanoma cells. The lot was composed of 30 male C57Bl6 mice, which received subcutaneous injections of 5x105 B16 melanoma cells into the right flank. Tumor volume was measured with a vernier caliper. Sialic acid was determined from the serum obtained by cardiac puncture. The second step of our research was performed on a number of 25 patients with cutaneous melanoma. Determination of sialic acid was performed using the Kattermann colorimetric method. The correlation between sialic acid and disease progression was exemplified in two clinical cases. Sialic acid determination was performed dynamically from diagnosis, following disease progression. Results: In murine models tumors increased after a lag period of up to 10 days. Tumor growth was recorded by measuring the tumor’s diameters and calculating its volume. We observed a progressive increase of sialic acid in parallel with tumor volume. In human subjects, sialic acid levels increase in metastatic disease and are common in localized disease. In the two clinical cases there was a very strong correlation between sialic acid and disease progression. Conclusions: B16 melanoma cells are highly metastatic. Sialic acid level was increased in metastatic tumor animals compared to normal animals. Higher levels of sialic acid have been shown to correlate with the metastatic potential of tumor cells. For humans, determination of total serum sialic acid would be more useful for diagnosis of advanced melanoma stage rather than for early detection and screening.
机译：目的：鉴定唾液酸是要在实验模型中使用的肿瘤标志物。获得的数据将外推给人类，因此该标记物可用于临床实践。材料和方法：我们使用了B16黑色素瘤细胞。该批次由30只雄性C57B16小鼠组成，这些小鼠经皮下注射5x105 B16黑色素瘤细胞到右侧。用游标卡尺测量肿瘤体积。从通过心脏穿刺获得的血清中测定唾液酸。我们研究的第二步是对25例皮肤黑色素瘤患者进行的。唾液酸的测定使用Kattermann比色法进行。在两个临床病例中例证了唾液酸与疾病进展之间的相关性。疾病进展后，根据诊断动态地进行唾液酸测定。结果：在鼠模型中，在长达10天的滞后后，肿瘤增加。通过测量肿瘤的直径并计算其体积来记录肿瘤的生长。我们观察到唾液酸的逐渐增加与肿瘤体积平行。在人类受试者中，唾液酸水平在转移性疾病中增加并且在局部疾病中很常见。在这两个临床病例中，唾液酸与疾病进展之间存在非常强的相关性。结论：B16黑色素瘤细胞具有高度转移性。与正常动物相比，转移性肿瘤动物的唾液酸水平升高。高水平的唾液酸已被证明与肿瘤细胞的转移潜能相关。对于人类而言，测定血清总唾液酸对诊断晚期黑色素瘤阶段将比对早期检测和筛查更为有用。

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