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首页> 外文期刊>Online Journal of Public Health Informatics >C. burnetii Shedding Study In Domestic Animals in Georgia
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C. burnetii Shedding Study In Domestic Animals in Georgia

机译：佐治亚州家畜的C.burnetii脱落研究

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Objective Q fever is poorly understood in Georgia and its prevalence is largely underestimated in both humans and animals.One of the main goal of the project was shedding study in domestic animals – isolation of C. burnetii from suspected seropositive animal blood, milk samples. Introduction Q fever is a zoonotic bacterial disease resulting from infection by Coxiella burnetii . Domestic ruminants (cattle, sheep, and goats) are considered the main reservoir for the pathogen, which can also infect humans. Q fever is poorly understood in Georgia and its prevalence is largely underestimated in both humans and animals. In Georgia Q fever laboratory diagnostic was started and implemented at the Laboratory of the Ministry of Georgia (LMA) within GG20 ,,Prevalence, Epidemiological Surveillance, and Laboratory Analysis of Coxiella burnetii in Georgia’’. Methods LMA conducted Coxiella burnetii shedding evaluation in three specific farms from Kvemo Kartli (Tsalka, Dmanisi) and Mtskheta-Mtianeti (Dusheti). Seropositive cattle and small ruminants were sampled per week. Sampling lasted 7 weeks and totally 581 samples samples (blood, milk and swab) were tested. Testing were conducted in a BSL3 laboratory under BSL3 working conditions. ACCM medium was used (2XACCm-2 acidified Citrate Cysteine Medium PH-4.75G N NaOH). The samples were incubated at 37°C using CO 2. Results As a result of the study, one culture was bacteriologically isolated from seropositive cattle milk sample ( the sample was taken on the third week of the study in Beshtasheni farm, Tslka, Kvemo Kartli) and confirmed by Molecular biology (PCR). Conclusions The study confirmed Q fever existence in Georgia. Traditionally considered an obligate intracellular agent, the requirement to be grown in tissue culture cells, embryonated eggs, or animal hosts has made it difficult to isolate C. Burnetii strains. Within the study one culture was isolated from the seropositive animal milk sample that was collected in the third week of the study. shedding of Coxiella burnetii in milk by infected cows appeared to be the most frequent positive sample for the bacterium. ?
机译：佐治亚州对客观Q发热了解甚少，在人类和动物中普遍低估了其流行程度。该项目的主要目标之一是减少对家畜的研究-从疑似血清阳性动物血液，牛奶样本中分离出伯氏梭菌。简介Q发烧是一种由伯氏柯氏杆菌感染引起的人畜共患细菌性疾病。国内反刍动物（牛，羊和山羊）被认为是病原体的主要储存库，病原体也可以感染人类。佐治亚州对Q热的了解很少，在人类和动物中其发病率都被低估了。在佐治亚州，佐治亚州Q实验室的发烧实验室诊断工作已在GG20内的佐治亚州实验室（LMA）开始进行，并进行了流行，流行病学监测和佐治亚州柯氏杆菌的实验室分析。方法LMA在来自Kvemo Kartli（Tsalka，Dmanisi）和Mtskheta-Mtianeti（Dusheti）的三个特定农场进行了柯氏杆菌脱落评估。每周对血清阳性的牛和反刍动物进行采样。采样持续了7周，共测试了581个样品样本（血液，牛奶和棉签）。测试是在BSL3工作条件下的BSL3实验室中进行的。使用ACCM培养基（2XACCm-2酸化的柠檬酸半胱氨酸培养基PH-4.75G N NaOH）。样品在37°C下用CO 2孵育。结果研究结果是从血清阳性牛乳样品中分离出一种培养物（样品在研究的第三周在Beshtasheni农场，Tslka，Kvemo Kartli采集。 ），并由分子生物学（PCR）确认。结论该研究证实佐治亚州存在Q热。传统上被认为是专性的细胞内制剂，由于需要在组织培养细胞，有胚卵或动物宿主中生长，因此很难分离出梭状芽胞杆菌菌株。在研究中，从在研究的第三周收集到的血清阳性动物乳样品中分离出一种培养物。被感染的母牛在牛奶中脱落的柯氏杆菌似乎是该细菌最常见的阳性样品。 ？

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