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首页> 外文期刊>Open access Journal of Sports Medicine >PPARα gene variants as predicted performance-enhancing polymorphisms in professional Italian soccer players
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PPARα gene variants as predicted performance-enhancing polymorphisms in professional Italian soccer players

机译：PPARα基因变异是意大利专业足球运动员预测的增强表现的多态性

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Background: The PPARα gene encodes the peroxisome proliferator-activator receptor alpha, a central regulator of expression of other genes involved in fatty acid metabolism. The purpose of this study was to determine the prevalence of G allele of the PPARα intron 7 G/C polymorphism (rs4253778) in professional Italian soccer players. Methods: Sixty professional soccer players and 30 sedentary volunteers were enrolled in the study. Samples of venous blood were obtained at rest, in the morning, by conventional clinical procedures; blood serum was collected and total cholesterol, high-density lipoprotein cholesterol, low-density lipoprotein cholesterol, and triglycerides were measured. An aliquot of anticoagulant-treated blood was used to prepare genomic DNA from whole blood. The G/C polymorphic site in PPARα intron 7 was scanned by using the PCR-RFLP (polymerase chain reaction restriction fragment length polymorphism) protocol with TaqI enzyme. Results: We found variations in genotype distribution of PPARα polymorphism between professional soccer players and sedentary volunteers. Particularly, G alleles and the GG genotype were significantly more frequent in soccer players compared with healthy controls (64% versus 48%). No significant correlations were found between lipid profile and genotype background. Conclusion: Previous results demonstrated an association of intron 7 G allele as well as the GG genotype in endurance athletes. Our result suggests that this is the case also in professional soccer players.
机译：背景：PPARα基因编码过氧化物酶体增殖物激活剂受体α，这是参与脂肪酸代谢的其他基因表达的中央调节剂。这项研究的目的是确定意大利专业足球运动员中PPARα内含子7 G / C多态性（rs4253778）的G等位基因的患病率。方法：60名职业足球运动员和30名久坐的志愿者参加了研究。通过常规临床程序，在早晨休息时获得静脉血样本。收集血清并测量总胆固醇，高密度脂蛋白胆固醇，低密度脂蛋白胆固醇和甘油三酸酯。用抗凝剂处理的血液的等分试样从全血制备基因组DNA。通过使用TaqI酶的PCR-RFLP（聚合酶链反应限制片段长度多态性）方案扫描PPARα内含子7中的G / C多态性位点。结果：我们发现职业足球运动员和久坐的志愿者之间PPARα多态性的基因型分布存在差异。特别是，与健康对照组相比，足球运动员中的G等位基因和GG基因型显着更高（64％对48％）。在脂质谱与基因型背景之间未发现显着相关性。结论：先前的结果表明，耐力运动员中内含子7 G等位基因与GG基因型相关。我们的结果表明，职业足球运动员也是如此。

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