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首页> 外文期刊>Open access Insect Physiology >Low temperature induced alterations in certain biochemical constituents of 5th instar larvae of Philosamia ricini (Lepidoptera: Satunidae)
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Low temperature induced alterations in certain biochemical constituents of 5th instar larvae of Philosamia ricini (Lepidoptera: Satunidae)

机译：低温诱导的百日红5龄幼虫某些生化成分发生变化（鳞翅目：Sa科）

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Abstract: Studies on cold acclimation of insects including silkworms have shown significant variations in the levels of various biomolecules to cope with heat-shock. The present study has been carried out on cold-stressed 5th instar Philosamia ricini Hutt larvae to assess the physiological strategy adopted by this silkworm species for its survival at low temperatures. In this study, various biochemical assays have been performed in hemolymph, silk gland, and fat body of the larvae. The results revealed that exposure to low temperatures (10°C ± 1°C) leads to the accumulation of high levels of glycerol content in hemolymph, silk gland, and fat body of larvae in comparison with control group reared at ambient temperature conditions (25°C ± 3°C). The treatment of P. ricini larvae with low temperatures resulted in decreased trehalose content in all 3 insect tissues tested. The activities of phosphatases (acid and alkaline) were found to be significantly increased. However, the activities of aminotransferases (L-alanine-2-oxoglutarate aminotransferase [GPT, EC: 2.6.1.2] and L-aspartate-2-oxoglutarate aminotransferase [GOT, EC: 2.6.1.1]) were significantly inhibited in the hemolymph. The data indicated that trehalose could function as the major carbohydrate source of energy in acclimated 5th instar P. ricini larvae, whereas glycerol appears to confer cryoprotection.
机译：摘要：对包括蚕在内的昆虫进行冷驯化的研究表明，应对高温冲击的各种生物分子的含量存在显着差异。本研究已在冷应激的5龄幼虫Philosamia ricini Hutt幼虫上进行，以评估该蚕种在低温下生存所采用的生理策略。在这项研究中，已在幼虫的血淋巴，丝腺和脂肪体中进行了各种生化分析。结果显示，与在环境温度条件下饲养的对照组相比，暴露于低温（10°C±1°C）会导致幼虫的血淋巴，丝腺和幼虫脂肪体内甘油含量高水平的积累（25 °C±3°C）。低温处理蓖麻假单胞菌幼虫会导致所测试的所有3种昆虫组织中海藻糖含量降低。发现磷酸酶的活性（酸和碱）显着增加。但是，在血淋巴中，氨基转移酶（L-丙氨酸-2-氧代谷氨酸氨基转移酶[GPT，EC：2.6.1.2]和L-天冬氨酸-2-氧代谷氨酸氨基转移酶[GOT，EC：2.6.1.1]）的活性被显着抑制。数据表明，海藻糖可以作为适应环境的5龄幼虫P. ricini幼虫的主要碳水化合物能源，而甘油似乎具有冷冻保护作用。

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