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首页> 外文期刊>Oncogene >Regulation of Bcl-2 expression by C|[sol]|EBP in t(14;18) lymphoma cells
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Regulation of Bcl-2 expression by C|[sol]|EBP in t(14;18) lymphoma cells

机译：C | [sol] | EBP对t（14; 18）淋巴瘤细胞中Bcl-2表达的调节

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In follicular lymphomas with the t(14;18) translocation, there is increased expression of the bcl-2 gene, which is dependent upon regulatory elements within the bcl-2 5' flanking region and the immunoglobulin heavy-chain gene enhancers. We found that t(14;18) lymphomas expressed C/EBP, which is not normally expressed in B lymphocytes. Expression of C/EBP increased bcl-2 expression, and two regions of the bcl-2 P2 promoter that mediated this effect were identified. C/EBP was also able to increase bcl-2 promoter activity through these sites. The 5' site was GC-rich and did not contain a C/EBP consensus sequence; however, C/EBP was observed to interact with this site both in vitro by EMSA and in vivo by chromatin immunoprecipitation assay. The 3' region contained the Cdx site, which mediates the effect of A-Myb on the bcl-2 promoter. In vivo binding studies revealed that C/EBP interacted with this region of the bcl-2 promoter as well. Decreased expression of C/EBP factors due to targeting of their transcripts by siRNA molecules resulted in downregulation of Bcl-2 protein. We conclude that C/EBP and C/EBP contribute to the deregulated expression of Bcl-2 in t(14;18) lymphoma cells.
机译：在具有t（14; 18）易位的滤泡性淋巴瘤中，bcl-2基因的表达增加，这取决于bcl-2 5'侧翼区域和免疫球蛋白重链基因增强子内的调控元件。我们发现t（14; 18）淋巴瘤表达C / EBP，而在B淋巴细胞中通常不表达。 C / EBP的表达增加bcl-2表达，并鉴定了介导此作用的bcl-2 P2启动子的两个区域。 C / EBP还能够通过这些位点增加bcl-2启动子的活性。 5'位点富含GC，不包含C / EBP共有序列。然而，通过EMSA在体外和通过染色质免疫沉淀测定法观察到C / EBP均与该位点相互作用。 3'区域包含Cdx位点，其介导A-Myb对bcl-2启动子的作用。体内结合研究表明，C / EBP也与bcl-2启动子的这一区域相互作用。由于siRNA分子靶向转录物，导致C / EBP因子表达降低，导致Bcl-2蛋白下调。我们得出结论，C / EBP和C / EBP有助于t（14; 18）淋巴瘤细胞中Bcl-2的表达失控。

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