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首页> 外文期刊>Sao Paulo Medical Journal >Model of human epidermis reconstructed in vitro with keratinocytes and melanocytes on dead de-epidermized human dermis
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Model of human epidermis reconstructed in vitro with keratinocytes and melanocytes on dead de-epidermized human dermis

机译：在死去表皮的人真皮上用角质形成细胞和黑素细胞体外重建人表皮模型

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CONTEXT: Recent progress in the field of epithelial culture techniques has allowed the development of culture systems in which the reconstructed epidermis presents characteristics of morphological differentiation similar to those seen in vivo. Human epidermis reconstructed in vitro may be used as the best alternative for the in vitro testing of the toxicology and efficiency of products for topical use, as well as in the treatment of skin burns and chronic skin ulcers. OBJECTIVE: To demonstrate a method for obtaining human epidermis reconstructed in vitro, using keratinocytes and melanocytes cultivated on dead de-epidermized human dermis. TYPE OF STUDY: Experimental/laboratory. SETTING: Skin Cell Culture Laboratory of the Faculdade de Ciências Médicas, Universidade Estadual de Campinas, Campinas, S?o Paulo, Brazil. PROCEDURE: Human keratinocytes and melanocytes cultured in vitro were grown on a biological matrix (dead de-epidermized human dermis) and the system was kept at an air-liquid interface, in a suitable culturing medium, until a stratified human epidermis was formed, maintaining the histological characteristics of the epidermis in vivo. RESULTS: It was histologically demonstrated that it is possible to reproduce a differentiated epidermis through keratinocytes and melanocytes cultured on dead de-epidermized human dermis, thus obtaining a correctly positioned human epidermis reconstructed in vitro with functional keratinocytes and melanocytes that is similar to in vivo epidermis. CONCLUSIONS: It is possible to obtain a completely differentiated human epidermis reconstructed in vitro from keratinocyte and melanocyte cultures on a dead de-epidermized human dermis.
机译：背景：上皮培养技术领域的最新进展允许培养系统的发展，其中重建的表皮呈现出与体内相似的形态分化特征。体外重建的人表皮可以用作体外测试局部使用产品的毒理学和功效以及治疗皮肤烧伤和慢性皮肤溃疡的最佳替代品。目的：展示一种利用在死去表皮化的人真皮上培养的角质形成细胞和黑素细胞来获得体外重建的人表皮的方法。研究类型：实验/实验室。地点：巴西圣保罗坎皮纳斯大学学院的皮肤细胞培养实验室。程序：将体外培养的人角质形成细胞和黑素细胞生长在生物基质上（去死皮表皮的人真皮），并在合适的培养基中将其保持在气液界面，直到形成分层的人表皮，并保持体内表皮的组织学特征结果：从组织学上证明，可以通过在死去表皮的人真皮上培养的角质形成细胞和黑素细胞繁殖分化的表皮，从而获得功能正确的人表皮，其功能性角质形成细胞和黑素细胞与体内表皮相似。结论：有可能从死去表皮的人真皮上的角质形成细胞和黑素细胞培养物中获得体外重建的完全分化的人表皮。

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