Post-mortem forensic identity testing: application of PCR to the identification of fire victim

Ana Elisa Correia Billerbeck; Daniel Romero Mu?oz; Edna Sadayo Miazato Iwamura; José Arnaldo Soares-vieira; Laís De Almeida Cardoso

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Post-mortem forensic identity testing: application of PCR to the identification of fire victim

机译：验尸法医鉴定测试：PCR在鉴定火灾受害者中的应用

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CONTEXT: DNA analysis has been used with success in the identification of carbonized corpses and victims of large accidents. The analysis requires relatives of crash victims to donate blood for analysis. The relatives are generally willing contribute to the identification by giving a blood sample. OBJECTIVE: To describe the use of the polymerase chain reaction (PCR) for genetic characterization of one victim extensively burned by fire. DESIGN: Case report. CASE REPORT: DNA was extracted from blood of the cardiac chamber, and 15 different loci (D1S80, ApoB, D17S30, D3S1744, D18S849, D12S1090, FGA, D7S820, D1S533, D9S304, HUMCSF1PO, HUMTPOX, HUMTHO1, amelogenin and HLA-DQA1) were analyzed using the PCR technique. Results from all loci typing of the corpse were then compared to that of his alleged biological parents, revealing a genetic compatibility.

机译：背景：DNA分析已成功用于鉴定碳化尸体和大事故受害者。分析要求坠毁受害者的亲戚捐献血液进行分析。亲戚通常愿意通过提供血液样本来帮助进行身份识别。目的：描述聚合酶链反应（PCR）在大范围烧伤的一名受害者的遗传特征鉴定中的应用。设计：病例报告。病例报告：DNA提取自心脏腔室血液和15个不同基因座（D1S80，ApoB，D17S30，D3S1744，D18S849，D12S1090，FGA，D7S820，D1S533，D9S304，HUMCSF1PO，HUMTPOX，HUMTHO1，使用PCR技术进行分析。然后将尸体所有基因座分型的结果与他所谓的亲生父母的结果进行比较，揭示了遗传相容性。

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《Sao Paulo Medical Journal》 |2000年第3期|共3页
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Ana Elisa Correia Billerbeck; Daniel Romero Mu?oz; Edna Sadayo Miazato Iwamura; José Arnaldo Soares-vieira; Laís De Almeida Cardoso;
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Post-mortem forensic identity testing: application of PCR to the identification of fire victim

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