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首页> 外文期刊>Science Advances >Tunable and label-free virus enrichment for ultrasensitive virus detection using carbon nanotube arrays
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Tunable and label-free virus enrichment for ultrasensitive virus detection using carbon nanotube arrays

机译：可调谐且无标签的病毒富集，可使用碳纳米管阵列进行超灵敏病毒检测

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Viral infectious diseases can erupt unpredictably, spread rapidly, and ravage mass populations. Although established methods, such as polymerase chain reaction, virus isolation, and next-generation sequencing have been used to detect viruses, field samples with low virus count pose major challenges in virus surveillance and discovery. We report a unique carbon nanotube size-tunable enrichment microdevice (CNT-STEM) that efficiently enriches and concentrates viruses collected from field samples. The channel sidewall in the microdevice was made by growing arrays of vertically aligned nitrogen-doped multiwalled CNTs, where the intertubular distance between CNTs could be engineered in the range of 17 to 325 nm to accurately match the size of different viruses. The CNT-STEM significantly improves detection limits and virus isolation rates by at least 100 times. Using this device, we successfully identified an emerging avian influenza virus strain [A/duck/PA/02099/2012(H11N9)] and a novel virus strain (IBDV/turkey/PA/00924/14). Our unique method demonstrates the early detection of emerging viruses and the discovery of new viruses directly from field samples, thus creating a universal platform for effectively remediating viral infectious diseases.
机译：病毒性传染病可能无法预测地爆发，迅速蔓延并破坏大量人口。尽管已使用诸如聚合酶链反应，病毒分离和下一代测序之类的既定方法来检测病毒，但是病毒计数低的田间样品在病毒监视和发现方面提出了重大挑战。我们报告了一种独特的碳纳米管尺寸可调富集微设备（CNT-STEM），它可以有效富集和浓缩从现场样品中收集的病毒。微型设备中的通道侧壁是通过生长垂直排列的氮掺杂多壁CNT的阵列制成的，其中CNT之间的管间距离可以设计在17至325 nm的范围内，以精确匹配不同病毒的大小。 CNT-STEM可以将检测极限和病毒分离率至少提高100倍。使用此设备，我们成功地鉴定了新兴的禽流感病毒株[A / duck / PA / 02099/2012（H11N9）]和新型病毒株（IBDV / turkey / PA / 00924/14）。我们独特的方法展示了对早期病毒的早期检测以及直接从田间样品中发现新病毒的能力，从而创建了一个有效补救病毒感染性疾病的通用平台。

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