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首页> 外文期刊>Shahid Beheshti University of Medical Sciences >Study of Antibacterial Activity of Ethylacetate Total Extract and the Alkaloid Fraction from Flowering Aerial Parts of Glaucium vitellinum Boiss. et Buhse against Clinical Isolates of Staphylococcus Spp.
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Study of Antibacterial Activity of Ethylacetate Total Extract and the Alkaloid Fraction from Flowering Aerial Parts of Glaucium vitellinum Boiss. et Buhse against Clinical Isolates of Staphylococcus Spp.

机译：Bo花开花地上部乙酸乙酯总提取物的抗菌活性和生物碱成分的研究。等人针对葡萄球菌属的临床分离株。

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Background: The purpose of this study was evaluation of the antimicrobial effect of ethyl acetate extract and alkaloid fraction of Glaucium vitellinum (G. vitellinum) against clinical Staphylococcus spp. isolates from patients of Sina hospital of Tehran. M aterials and Methods: The plant of G. vitellinum was collected from Khonsar, Isfahan province, during May 2014. It’s flowering and aerial parts were washed, dried, powdered and extracted with methanol and ethyl acetate by using percolator apparatus, separately. In continuation, the alkaloid fraction was separated from metanolic total extract. 100 clinical isolates of Staphylococcus spp . were collected randomly from different clinical samples of patients who referred to Sina hospital of Tehran during 2013-2014. Also, their resistant to common antibiotics were evaluated by disk diffusion method based on the CLSI 2014 protocol. Continuously, the antibacterial effect of ethyl acetate total extract and the alkaloid fraction against clinical isolates of Staphylococcus spp . were evaluated by determining the minimal inhibitory concentration (MIC) by microdilution method based on the CLSI 2014. Standard Staphylococcus aureus (PTCC1431) and Staphylococcus epidermidis (PTCC 1435) were evaluated, simultaneously. R es ults: Based on the results, 93% of isolates were coagulase positive and 7% were coagulase negative Staphylococci spp. (CoNS). All coagulase positive cocci were identified as Staphylococcus aureus and among 7% CoNS, 3% were identified as S. epidermidis , so, in the follow they are named only CoNS. The MIC of alkaloid fraction of G.vitellinum was: 17.87 mg/ml and 23.21 mg/ml against coagulase positive S.aureus and CoNS isolates , respectively . Also, MIC of ethyl acetate total extract of G.vitellinum was: 73.25 mg/ml and 98.21mg/ml on coagulase positive S. aureus and CoNS isolates, respectively . 61.29% of clinical S. aureus isolates were sensitive to ethyl acetate total extract? and 100 % were sensitive to alkaloid fraction while 100% were penicillin resistant while only 60%? of them were Trimethoprim/sulfamethoxazole (SXT) sensitive. Similarly, among CoNS isolates, 42.85% and 100% were sensitive to ethyl acetate total extract and alkaloid fraction, respectively. While 100% were penicillin resistant and only 42%were ciprofloxacin and doxycycline sensitivity. C onclusion: based on the existence of good antibacterial effect for alkaloid portion of G. vitellinum against clinical isolates of Staphylococcus spp. doing other in vitro and in vivo complementation tests are recommended for the further studies. ? Keyword: ? Glaucium vitellinum , Anti bacterial, Alkaloids, Papaveraceae ?
机译：背景：这项研究的目的是评估乙酸乙酯提取物和玻璃质生物碱（G. vitellinum）对临床葡萄球菌的抑菌作用。从德黑兰的新浪医院患者中分离得到。材料与方法：2014年5月，从美国伊斯法罕省孔萨尔市收集到了G. vitellinum的植物。将其花朵和地上部分分别洗净，干燥，撒粉，并使用渗滤器分别用甲醇和乙酸乙酯萃取。继续地，从代谢总提取物中分离出生物碱部分。 100株金黄色葡萄球菌的临床分离株。从2013-2014年转诊至德黑兰新浪医院的患者的不同临床样本中随机抽取患者。此外，通过基于CLSI 2014协议的磁盘扩散法评估了它们对常见抗生素的抗性。连续地，乙酸乙酯总提取物和生物碱组分对葡萄球菌属的临床分离株具有抗菌作用。通过基于CLSI 2014的微量稀释法确定最小抑菌浓度（MIC）进行评估。同时评估标准金黄色葡萄球菌（PTCC1431）和表皮葡萄球菌（PTCC 1435）。结果：根据结果，分离株的93％为凝固酶阳性，而7％为凝固酶阴性葡萄球菌。 （CoNS）。所有凝固酶阳性球菌均鉴定为金黄色葡萄球菌，在7％的CoNS中，有3％的鉴定为表皮葡萄球菌，因此，在下文中，它们仅称为CoNS。 G.vitellinum生物碱的MIC分别为：针对凝固酶阳性的金黄色葡萄球菌和CoNS分离株的17.87 mg / ml和23.21 mg / ml。同样，对凝固酶阳性的金黄色葡萄球菌和CoNS分离株，G.vitellinum的乙酸乙酯总提取物的MIC分别为：73.25 mg / ml和98.21mg / ml。 61.29％的临床金黄色葡萄球菌分离株对乙酸乙酯总提取物敏感？和100％对生物碱部分敏感，而100％对青霉素耐药，而只有60％？它们对甲氧苄啶/磺胺甲恶唑（SXT）敏感。同样，在CoNS分离物中，分别对乙酸乙酯总提取物和生物碱组分敏感的分别为42.85％和100％。虽然100％对青霉素耐药，但仅42％对环丙沙星和强力霉素敏感。纳入：基于对玻璃假单胞菌生物碱部分对葡萄球菌属的临床分离株具有良好的抗菌作用。建议进行其他体外和体内互补试验，以进行进一步的研究。 ？关键字：玻璃质玻璃粉，抗菌，生物碱，罂粟科？

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