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首页> 外文期刊>Open Veterinary Journal >Effects of storage temperature on the quantity and integrity of genomic DNA extracted from mice tissues: A comparison of recovery methods
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Effects of storage temperature on the quantity and integrity of genomic DNA extracted from mice tissues: A comparison of recovery methods

机译：储存温度对从小鼠组织中提取的基因组DNA数量和完整性的影响：回收方法的比较

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Efficient extraction of genomic DNA (gDNA) from biological materials found in harsh environments is the first step for successful forensic DNA profiling. This study aimed to evaluate two methods for DNA recovery from animal tissues (livers, muscles), focusing on the best storage temperature for DNA yield in term of quality, quantity, and integrity for use in several downstream molecular techniques. Six male Swiss albino mice were sacrificed, liver and muscle tissues (n=32) were then harvested and stored for one week in different temperatures, -20oC, 4oC, 25oC and 40oC. The conditioned animal tissues were used for DNA extraction by Chelex-100 method or NucleoSpin? Blood and Tissue kit. The extracted gDNA was visualized on 1.5% agarose gel electrophoresis to determine the quality of gDNA and analysed spectrophotometrically to determine the DNA concentration and the purity. Both methods, Chelex-100 and NucleoSpin? Blood and Tissue kit found to be appropriate for yielding high quantity of gDNA, with the Chelex?100 method yielding a greater quantity (P < 0.045) than the kit. At -20oC, 4oC, and 25oC temperatures, the concentration of DNA yield was numerically lower than at 40oC. The NucleoSpin? Blood and Tissue kit produced a higher (P=0.031) purity product than the Chelex-100 method, particularly for muscle tissues. The Chelex-100 method is cheap, fast, effective, and is a crucial tool for yielding DNA from animal tissues (livers, muscles) exposed to harsh environment with little limitations.
机译：从恶劣环境中发现的生物材料中高效提取基因组DNA（gDNA）是成功进行法医DNA分析的第一步。这项研究旨在评估从动物组织（肝脏，肌肉）中回收DNA的两种方法，着眼于质量，数量和完整性方面可用于几种下游分子技术的最佳DNA储存温度。处死六只瑞士白化病雄性小鼠，然后收集肝脏和肌肉组织（n = 32）并在不同温度（-20oC，4oC，25oC和40oC）下保存一周。将经过条件处理的动物组织用于通过Chelex-100方法或NucleoSpin？DNA提取DNA。血液和组织试剂盒。提取的gDNA在1.5％琼脂糖凝胶电泳上可视化，以确定gDNA的质量，并用分光光度法进行分析，以确定DNA的浓度和纯度。 Chelex-100和NucleoSpin这两种方法？血液和组织试剂盒被发现适合于产生大量的gDNA，而Chelex？100方法所产生的数量要多于试剂盒（P <0.045）。在-20oC，4oC和25oC温度下，DNA产量的浓度在数值上低于40oC。 NucleoSpin？血液和组织试剂盒产生的纯度比Chelex-100方法高（P = 0.031），特别是对于肌肉组织。 Chelex-100方法便宜，快速，有效，是从暴露在恶劣环境中的动物组织（肝脏，肌肉）无限制地提取DNA的重要工具。

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