首页>
外国专利>
KIT FOR EXTRACTING GENOMIC DNA FROM HUMAN BLOOD AND METHOD FOR EXTRACTING GENOMIC DNA USING THE SAME
KIT FOR EXTRACTING GENOMIC DNA FROM HUMAN BLOOD AND METHOD FOR EXTRACTING GENOMIC DNA USING THE SAME
展开▼
机译：用于从人血中提取基因组DNA的试剂盒以及使用该试剂盒提取基因组DNA的方法
展开▼
页面导航
摘要
著录项
相似文献
摘要
The present invention using the kit, and it comprised of a reagent capable of extracting a genomic DNA from human blood relates to a method of extracting the genomic DNA from human blood, human blood was added to the non-solidified 20mM, pH 7.5 with Tris-HCl buffer solution for dissolving red blood cells comprising the kit, red blood cells, plasma, etc. to remove white blood cells and platelet components a first step for obtaining only; The resulting white blood cell to 10mM, Tris-HCl buffer of pH 8.0, the second step eluting solution was added to the nucleus of the white blood sets for 1.0mM EDTA and 1.0% made up of white blood cells dissolved SDS; 3M the nucleus of the white blood cells release a third step of separating the genomic DNA and nuclear proteins from the nucleus of the white blood cells was added to the protein solution, the kit comprising instructions only sodium acetate solution of pH 5.2; And genomic DNA from human blood made of a fourth step in the recovery of genomic DNA isolated genome DNA 10mM, pH 8.0 Tris-HCl buffer solution of genomic DNA, and 1.0mM EDTA recovering consisting of TE buffer was added to the kit in the It provides extraction methods.
展开▼
