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首页> 外文期刊>South African Journal of Animal Science >PCR-RFLP-based identification of polymorphisms in BMPR1B, GDF9 and BMP15 genes associated with litter size in Beetal and Teddy goats
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PCR-RFLP-based identification of polymorphisms in BMPR1B, GDF9 and BMP15 genes associated with litter size in Beetal and Teddy goats

机译：基于PCR-RFLP的BMPR1B，GDF9和BMP15基因多态性与牛和泰迪山羊产仔数相关的鉴定

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The genes BMPR1B, GDF9 and BMP15 are oocyte-derived members of the transforming growth factor-β superfamily, which is essential for follicular growth and ovulation. The aim of the current study was to determine the incidence of reported mutations in exon 3 of BMPR1B gene, exon 1 of GDF9 gene, and exon 2 of BMP15 gene and their association with litter size in three parities of Beetal and Teddy goats. Based on the known mutations in these genes, PCR primers were designed to find out the polymorphism through the PCR-RFLP technique in 120 randomly selected animals of the two breeds, which vary in their prolificacy. The current results show that there were significant differences in litter size [1.708 ± 0.060 in Beetal (B) and 2.167 ± 0.056 in Teddy (T) goats] in both breeds. The results of breed x parity interaction showed significant differences in litter size in first [1.158 ± 0.061 (B); 1.861 ± 0.057 (T)], third [2.062 ± 0.078 (B); 2.583 ± 0.073 (T)] and average parity [708 ± 0.060 (B); 2.167 ± 0.056 (T)] in both breeds. All three loci in both breeds were found to be polymorphic and the results of breed x parity x genotype analysis showed that genotypes of all the three genes were significantly associated with litter size in all parities in both breeds. For the BMPR1B gene, heterozygous (CT) animals of Beetal and Teddy goats had the largest litter size in all three parities and the average (parity) litter size, showing the overdominance of the heterozygotes. Similarly, for GDF9 gene, heterozygous animals (AG) had the largest litter size in both breeds. For BMP15 gene homozygous carrier animals (CC) of both breeds had the highest number of kids, followed by the heterozygous (AC) genotypes. The current results show the importance of BMPR1B, GDF9 and BMP15 as the major genes that influence prolificacy in both Beetal and Teddy goats. It is suggested that these polymorphisms can be used as molecular markers to select the prolific animals in these breeds.
机译：BMPR1B，GDF9和BMP15基因是卵母细胞来源的转化生长因子-β超家族，对卵泡生长和排卵至关重要。本研究的目的是确定在三只Beetal和Teddy山羊中，BMPR1B基因外显子3，GDF9基因外显子1和BMP15基因外显子2报道的突变发生率及其与产仔量的关系。基于这些基因中已知的突变，设计PCR引物，以通过PCR-RFLP技术在两个品种的120个随机选择的动物中发现多态性，这些动物的繁殖力各不相同。目前的结果表明，两个品种的产仔数存在显着差异[Betal（B）为1.708±0.060，Teddy（T）山羊为2.167±0.056]。品种x奇偶性相互作用的结果显示，第一胎产仔数差异显着[1.158±0.061（B）; 1.861±0.057（T）]，第三[2.062±0.078（B）; 2.583±0.073（T）]和平均奇偶校验[708±0.060（B）;在两个品种中为2.167±0.056（T）]。发现两个品种的所有三个基因座都是多态的，品种x均等x基因型分析的结果表明，这三个基因的所有三个基因型均与产仔数显着相关。对于BMPR1B基因，比塔尔山羊和泰迪山羊的杂合子（CT）动物在所有三个胎次中均具有最大的产仔数，而平均（胎次）产仔数则显示出杂合子的优势。同样，对于GDF9基因，杂合动物（AG）在两个品种中的窝产仔数最大。对于BMP15基因，两个品种的纯合子携带者（CC）的孩子数量最多，其次是杂合（AC）基因型。目前的结果表明BMPR1B，GDF9和BMP15作为影响Beetle和Teddy山羊繁殖能力的主要基因的重要性。建议将这些多态性用作选择这些品种中多产动物的分子标记。

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