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Complete genome sequence of Polynucleobacter necessarius subsp. asymbioticus type strain (QLW-P1DMWA-1T)

机译:完全必要的多核细菌亚种的全基因组序列。非共生型菌株(QLW-P1DMWA-1T)

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Polynucleobacter necessarius subsp. asymbioticus strain QLW-P1DMWA-1T is a planktonic freshwater bacterium affiliated with the family Burkholderiaceae (class Betaproteobacteria). This strain is of interest because it represents a subspecies with cosmopolitan and ubiquitous distribution in standing freshwater systems. The 16S-23S ITS genotype represented by the sequenced strain comprised on average more than 10% of bacterioplankton in its home habitat. While all strains of the subspecies P. necessarius asymbioticus are free-living freshwater bacteria, strains belonging to the only other subspecies, P. necessarius subsp. necessarius are obligate endosymbionts of the ciliate Euplotes aediculatus. The two subspecies of P. necessarius are the instances of two closely related subspecies that differ in their lifestyle (free-living vs. obligate endosymbiont), and they are the only members of the genus Polynucleobacter with completely sequenced genomes. Here we describe the features of P. necessarius subsp. asymbioticus, together with the complete genome sequence and annotation. The 2,159,490 bp long chromosome with a total of 2,088 protein-coding and 48 RNA genes is the first completed genome sequence of the genus Polynucleobacter to be published and was sequenced as part of the DOE Joint Genome Institute Community Sequencing Program 2006.
机译:必需多核杆菌亚种。非对称细菌QLW-P1DMWA-1T是浮游淡水细菌,隶属于伯克霍尔德氏菌科(Betaproteobacteria类)。该菌株引起人们的兴趣,因为它代表了在站立的淡水系统中具有世界性和普遍分布的亚种。由测序菌株代表的16S-23S ITS基因型在其家庭栖息地中平均包含超过10%的浮游细菌。尽管所有亚种P. necessarius asymbioticus菌株都是自由生存的淡水细菌,但属于唯一其他亚种P. necessarius亚种的菌株。必需品是纤毛Euplotes aediculatus的专性内共生体。 P. necessarius的两个亚种是两个生活方式密切相关的亚种的实例(自由生活与专性内共生),并且它们是多核细菌属中唯一具有完全测序基因组的成员。在这里,我们描述P. necessarius亚种的特征。不对称,以及完整的基因组序列和注释。这条长2,159,490 bp的染色体,共有2088个蛋白质编码和48个RNA基因,是多核细菌属中第一个完整的基因组序列,即将出版,并作为美国能源部联合基因组研究所社区测序计划2006的一部分进行了测序。

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