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首页> 外文期刊>Stem cells international >Therapeutic Effects of VEGF Gene-Transfected BMSCs Transplantation on Thin Endometrium in the Rat Model
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Therapeutic Effects of VEGF Gene-Transfected BMSCs Transplantation on Thin Endometrium in the Rat Model

机译：VEGF基因转染的骨髓间充质干细胞移植对大鼠子宫内膜薄层的治疗作用

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Objective. Bone mesenchymal stem cells (BMSCs) transplantation has a therapeutic effect on the thin endometrium in animal researches and clinical trials. The present study aims at assessing whether transplantation of VEGF-transfected BMSCs (VEGF-BMSCs) have a better therapeutic effect on endometrial regeneration and endometrial receptivity compared with BMSCs therapy alone. Methods. Sprague-Dawley (SD) rats were used in the study. Thin endometrium model was established with 95% ethanol injection into uterine. VEGF-BMSCs or BMSCs was transplanted via tail vein IV injection. Endometrial thickness, morphology, and pinopodes were assessed by hematoxylin and eosin (HE) staining and scanning electron microscope (SEM). The proteins and mRNAs expressions of markers for endometrial cells and endometrial receptivity were measured after treatment. The fertility testing was done to assess the embryo implantation efficiency. Results. VEGF-BMSCs transplantation significantly increased endometrial thickness compared with the BMSCs group and the control group. There was no significant difference in endometrial thickness between VEGF-BMSCs group and sham operation group. Importantly, in protein level, expressions of cytokeratin, vitamin, VEGF, LIF, and integrin ανβ3 in VEGF-BMSC group were increased dramatically compared with those of the control group and BMSC group both 4 days and 8 days after stem cells transplantation. Accordingly, mRNA expression of LIF and integrin ανβ3 was significantly upregulated compared with those of the control group and BMSC group both 4 and 8 days after treatment. The pinopodes were developed better in the VEGF-BMSCs group and the sham operation group compared with BMSCs group and the control group. The number of embryo implantation is largest in the sham operation group, followed by VEGF-BMSCs group, BMSCs group, and the control group. Conclusions. Transplantation of VEGF gene-transfected BMSCs may be a better therapeutic treatment for thin endometrium than stem cell therapy alone.
机译：目的。在动物研究和临床试验中，骨间充质干细胞（BMSCs）移植对子宫内膜薄有治疗作用。本研究旨在评估与单独的BMSCs治疗相比，移植VEGF的BMSCs（VEGF-BMSCs）移植对子宫内膜再生和子宫内膜容受性是否具有更好的治疗效果。方法。研究中使用了Sprague-Dawley（SD）大鼠。子宫内膜稀薄模型通过向子宫内注射95％乙醇建立。通过尾静脉IV注射移植VEGF-BMSC或BMSC。通过苏木精和曙红（HE）染色和扫描电子显微镜（SEM）评估子宫内膜的厚度，形态和羽状十二指肠。处理后测定子宫内膜细胞标志物的蛋白质和mRNA表达以及子宫内膜的接受性。进行生育力测试以评估胚胎植入效率。结果。与BMSCs组和对照组相比，VEGF-BMSCs移植显着增加了子宫内膜厚度。 VEGF-BMSCs组与假手术组子宫内膜厚度无明显差异。重要的是，在蛋白质水平上，干细胞移植后4天和8天，VEGF-BMSC组中细胞角蛋白，维生素，VEGF，LIF和整联蛋白ανβ3的表达均显着高于对照组和BMSC组。因此，在处理后4天和8天，与对照组和BMSC组相比，LIF和整联蛋白ανβ3的mRNA表达显着上调。与BMSCs组和对照组相比，VEGF-BMSCs组和假手术组的梭极发育更好。在假手术组中，胚胎植入的数量最多，其次是VEGF-BMSCs组，BMSCs组和对照组。结论。 VEGF基因转染的骨髓间充质干细胞的移植可能比单独的干细胞疗法更好。

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