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A study on the protective effect of bone marrow derived mesenchymal stem cells on chronic renal failure in rats

机译：骨髓间充质干细胞对大鼠慢性肾功能衰竭的保护作用研究

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Chronic renal failure (CRF) is a common disease with high morbidity and mortality. The inadequacy of current treatment modalities and insufficiency of donor organs for cadaveric transplantation have driven a search for improved methods of dealing with renal failure. This raises the concept of cell-based therapy using mesenchymal stem cells (MSCs). The purpose of this work is to evaluate the effect of MSCs in rats with CRF and to detect the level of B-cell lymphoma 2 (Bcl-2) and basic fibroblast growth factor (bFGF) as possible antiapoptotic and anti-inflammatory paracrine mediators of MSCs action. This study was carried on female albino rats, which were divided into 3 groups, control group, CRF group and CRF/MSCs group. MSCs were derived from bone marrow of male albino rats and were characterized morphologically and by RT-PCR for CD29. Serum urea, creatinine, Na, K, systolic blood pressure and 24-hour urinary albumin were estimated for all groups, Y-chromosome gene (Sry) was detected by PCR in renal tissue. Also Bcl-2 and bFGF were examined by RT-PCR in renal tissue of all studied groups and the kidney was examined pathologically. The results of this work showed that CRF rats receiving MSCs showed significantly lowered serum urea, creatinine and urinary albumin levels compared to the CRF group. Also improvement of serum Na and K was observed in CRF/MSCs group compared to CRF group. As regard Bcl-2 and bFGF they were highly expressed in renal tissue of CRF/MSCs group compared to the CRF group. The (Sry) gene was detected by PCR in the renal tissue of CRF/MSCs group. These results demonstrate a potential of MSCs to ameliorate the kidney function in rats with CRF possibly through the antiapoptotic and anti-inflammatory paracrine mediators of MSCs.
机译：慢性肾衰竭（CRF）是一种高发病率和高死亡率的常见疾病。当前治疗方法的不足和尸体移植的供体器官不足，促使人们寻求治疗肾衰竭的改良方法。这就提出了使用间充质干细胞（MSC）进行基于细胞的治疗的概念。这项工作的目的是评估MSCs在CRF大鼠中的作用，并检测B细胞淋巴瘤2（Bcl-2）和碱性成纤维细胞生长因子（bFGF）的水平，作为可能的抗凋亡和抗炎性旁分泌介质。 MSC动作。本研究对雌性白化病大鼠进行了实验，将其分为3组，对照组，CRF组和CRF / MSCs组。 MSC来自白化病雄性大鼠的骨髓，并通过RT-PCR对CD29进行形态学表征。估计所有组的血清尿素，肌酐，Na，K，收缩压和24小时尿白蛋白，并通过PCR在肾脏组织中检测Y染色体基因（Sry）。还通过RT-PCR在所有研究组的肾脏组织中检查了Bcl-2和bFGF，并且对肾脏进行了病理检查。这项工作的结果表明，与CRF组相比，接受MSC的CRF大鼠的血清尿素，肌酐和尿白蛋白水平显着降低。与CRF组相比，CRF / MSCs组也观察到血清Na和K的改善。关于Bcl-2和bFGF，与CRF组相比，它们在CRF / MSCs组的肾组织中高表达。通过PCR检测CRF / MSCs组肾脏组织中的（Sry）基因。这些结果表明，MSCs有可能通过MSCs的抗凋亡和抗炎性旁分泌介质改善CRF大鼠的肾功能。

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