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Protein quantitation using iTRAQ: Review on the sources of variations and analysis of nonrandom missingness

机译:使用iTRAQ进行蛋白质定量:综述变异来源和非随机缺失

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As a technique that allows simultaneous quantitation of proteins in multiple samples, iTRAQ (isobaric Tags for Relative and Absolute Quantitation) has gained increased interest and applications in proteomics research. Despite its success, iTRAQ data present a number of statistical challenges even after the proteins and peptides are identified and the peak areas of the reported ions are estimated for peptide intensities. In this article, we review recent studies on the analysis of iTRAQ data, the computation problems involved and the nonrandom missingness in the iTRAQ data.
机译:作为允许同时定量多个样品中蛋白质的技术,iTRAQ(相对定量和绝对定量的等压标记)在蛋白质组学研究中获得了越来越多的关注和应用。尽管取得了成功,iTRAQ数据仍然存在许多统计难题,即使在鉴定了蛋白质和肽段并估算了报告离子的峰面积后也得出了肽段强度。在本文中,我们回顾了有关iTRAQ数据分析,涉及的计算问题以及iTRAQ数据中的非随机缺失的最新研究。

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