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首页> 外文期刊>Spectroscopy >Parallel folding pathway of proline-free staphylococcal nuclease studied by the stopped-flow double-jump method
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Parallel folding pathway of proline-free staphylococcal nuclease studied by the stopped-flow double-jump method

机译：停止流双跳跃法研究无脯氨酸葡萄球菌核酸酶的平行折叠途径

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摘要

The folding mechanism of proline?free staphylococcal nuclease (SNase (pro?)) (P11A, P31A, P42A, P47T, P56A, P117G) was investigated using the double?jump stopped?flow method (interrupted refolding). This method has enabled us to specifically monitor the amount of the native molecules during the refolding. The results indicate that the middle and slow phases observed in the refolding kinetics represent the formation of the native state (IM→N, IS→N) and that the folding mechanism of SNase (pro?) is not represented by a single sequential pathway, but at least two parallel pathways are required for interpreting the results.
机译：采用双跳停流法（间断重折叠）研究了无脯氨酸的葡萄球菌核酸酶（SNase（prol））（P11A，P31A，P42A，P47T，P56A，P117G）的折叠机制。这种方法使我们能够在重新折叠过程中专门监测天然分子的数量。结果表明，在重折叠动力学中观察到的中间阶段和缓慢阶段代表了天然状态的形成（IM→N，IS→N），并且SNase（pro？）的折叠机制并没有由单一的顺序途径表示，但至少需要两条平行的路径才能解释结果。

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