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首页> 外文期刊>Pakistan journal of botany >Evaluation of genetic diversity in different Pakistani wheat land races
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Evaluation of genetic diversity in different Pakistani wheat land races

机译：不同巴基斯坦小麦陆族遗传多样性的评估

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摘要

Wheat is one of the main sources of nutrition worldwide. Genetic improvement of the seed makes wheat a source of high quality flour for human consumption and for other industrial uses. With the help of molecular markers, the available germplasm of wheat can be assessed for future breeding programs. Therefore, the aim of the present work was to analyze the genetic diversity among 15 Pakistani wheat land races based on Random Amplified Polymorphism DNA (RAPD) markers. A total of 284 DNA fragments were amplified, ranging in size from 200bp to 1100bp by using six primers. The number of DNA fragments for each primer varied from 2 (OPC-6) to 9 (OPC-8) with an average of 6 fragments per primer. Out of 284 amplified products, 120 were monomorphic and 137 were polymorphic showing an average of 7.8% polymorphism per primer. One specific marker was detected both for OPC-1 and OPC-8, two for OPC-5, while no RAPD specific marker was detected for the remaining primers. The genetic similarity index values ranged from 0.36 to 0.93, with an average of 0.64. Maximum genetic similarity (91%) was observed between Sur bej (3) and Khushkawa (11). On the contrary, minimum genetic similarity (32%) was observed in Khushkaba-1 (5) and Khushkawa (11). The dendrogram resulting from the NTSYS cluster analysis showed that the studied genotypes are divided into two main clusters from the same node. The first cluster contained 13 land races, while the second cluster contained only 2 land races. The dendrogram clustered the genotypes into 5 groups and showed efficiency in identifying genetic variability. These results indicated the usefulness of RAPD technique in estimating the genetic diversity among wheat genetic resources.
机译：小麦是全世界主要的营养来源之一。种子的遗传改良使小麦成为人类食用和其他工业用途的优质面粉的来源。借助分子标记，可以评估小麦的可用种质，以用于将来的育种计划。因此，本研究的目的是基于随机扩增多态性DNA（RAPD）标记分析15个巴基斯坦小麦陆族之间的遗传多样性。通过使用六个引物，总共扩增了284个DNA片段，大小从200bp到1100bp不等。每个引物的DNA片段数从2（OPC-6）到9（OPC-8）不等，每个引物平均6个片段。在284个扩增产物中，有120个是单态的，有137个是多态的，每个引物平均有7.8％的多态性。 OPC-1和OPC-8均检测到一种特异性标记，OPC-5均检测出两种，而其余引物未检测到RAPD特异性标记。遗传相似性指数值介于0.36至0.93之间，平均值为0.64。在Sur bej（3）和Khushkawa（11）之间观察到最大的遗传相似性（91％）。相反，在Khushkaba-1（5）和Khushkawa（11）中观察到最小的遗传相似性（32％）。 NTSYS聚类分析得出的树状图显示，研究的基因型从同一节点分为两个主要的聚类。第一组包含13个陆地种族，而第二组仅包含2个陆地种族。树状图将基因型分为5组，并显示了鉴定遗传变异性的效率。这些结果表明，RAPD技术在估算小麦遗传资源之间的遗传多样性方面很有用。

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