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首页> 外文期刊>Pakistan journal of botany >Differential responses of photosystem II activity to photooxidation in red and green tissues of Amaranthus tricolor leaves
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Differential responses of photosystem II activity to photooxidation in red and green tissues of Amaranthus tricolor leaves

机译:三色leaves叶的红色和绿色组织中光系统II活性对光氧化的差异响应

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摘要

In order to study the antioxidative potential of amaranthine and its relationships with photoprotection, changes of PS IIactivity of red and green tissue in the same edible amaranth leaf were compared under photooxidation treatment induced by MV(methyl viologen) or H2O2. In the first 90 min of MV treatment, PS II maintained stable activity; the chlorophyll fluorescenceparameters were close to those of the controls. However, with the extension of treatment time, during 90 300 min of continuousphotooxidative stress, Fv/Fm, Y (II) and Y (NPQ) of leaf discs decreased significantly, while Y (NO) and Fodramaticallyincreased, which showed that the activity of PS II suffered irreversible photooxidative damage, suppressed or even completelyinhibited. Compared with the control, under 50 mmol·L1of H2O2stress for 360 min, Y(NO) and Foincreased, accompanied byY(NPQ) and the Fv/Fmdecreases, while Y(II), qP and ETR were slightly increased, reflecting that treatment with 50 mmol·L1H2O2promoted distribution of light energy in photochemistry reaction. After photooxidative treatments, a lower Foand Y (NO)but higher Fv/Fm, Y (II), qP and Y (NPQ) was found in the red leaf discs compared with the green ones. The decrease and thedecline rate of the amaranthine content in the green and red leaf discs implied that the PS II of green leaf discs have a highersensitivity to photooxidative damage than the red ones. It is suggested that the higher content of amaranthine may be used as adetoxification mechanism to counteract photooxidation in red leaf.
机译:为了研究of菜红素的抗氧化能力及其与光保护作用的关系,比较了MV(甲基紫精)或H2O2诱导的同一氧化edible菜叶片中红色和绿色组织中PS和PS II活性的变化。在MV处理的前90分钟,PS II保持稳定的活性。叶绿素荧光参数接近于对照。然而,随着处理时间的延长,在连续光氧化胁迫的90 300 min内,叶盘的Fv / Fm,Y(II)和Y(NPQ)显着下降,而Y(NO)和Fodramatically增加,表明PS II遭受不可逆的光氧化损伤,被抑制甚至完全被抑制。与对照相比,在50mmol·L1的H2O2胁迫下持续360 min,Y(NO)和Fo增加,伴随Y(NPQ)和Fv / Fm减少,而Y(II),qP和ETR略有增加,反映了50mmol·L1H2O2促进光化学反应中光能的分布。经过光氧化处理后,与绿色的相比,红叶盘中的Foand Y(NO)较低,但Fv / Fm,Y(II),qP和Y(NPQ)较高。绿叶盘和红叶盘中的mar菜红素含量的下降和下降速率表明,绿叶盘的PS II对光氧化损伤的敏感性高于红色叶盘。提示较高含量的mar菜红可以作为抗氧化作用的抗氧化机理。

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