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首页> 外文期刊>Pakistan journal of botany >Construction of microsatellite linkage map and detection of segregation distortion in Indica rice (Oryza sativa L.)
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Construction of microsatellite linkage map and detection of segregation distortion in Indica rice (Oryza sativa L.)

机译：rice稻微卫星连锁图的构建及偏析的检测

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High density molecular linkage map is a prerequisite to identify the quantitative traits loci. Construction of linkage maps, using mapping populations derived from two major subspecies: indica and japonica in rice, have been reported. However, limited work was conducted in indica subspecies. Present studies focused to construct a molecular map based on indica × indica (Shaheen Basmati × Pokkali) derived F2 mapping population. The detected microsatellite polymorphism was only 50.64% between parental cultivars. A total of 107 microsatellite markers were employed to amplify 108 loci, distributed throughout the genome. The marker data of 190 individuals was recorded for map construction. Most of the markers were found co-dominant, where as eight markers exhibited dominance in favor of Shaheen Basmati alleles and four in favor of Pokkali alleles. Detected segregation distortion was 7.47%, which was significantly low than previous studies. MapMaker was used to construct the linkage groups. The map spanned 1753.9 cM (Kosambi function) with microsatellite markers on 12 rice chromosomes and an average distance of 16.2 cM between markers. This map contained a greater and least percentage of markers on linkage group 3 and 12, respectively. Maximum common interval size (MCIS) analysis revealed that present map (indica × indica) covered the rice genome 98% of previous investigation (japonica × indica). Because of diverse background of parental cultivars, low segregation distortion and high genome coverage, revealed that molecular linkage map would be used as marker framework to investigate the genetics of important agronomic traits in indica rice.
机译：高密度分子连锁图谱是鉴定数量性状基因座的前提。已经报道了利用来自两个主要亚种：水稻中的in稻和粳稻的作图种群构建连锁图。但是，在in亚种中进行的工作有限。目前的研究集中在构建基于×××（Shaheen Basmati×Pokkali）的F2作图群体的分子图。亲本品种之间检测到的微卫星多态性仅为50.64％。总共使用107个微卫星标记来扩增分布在整个基因组中的108个基因座。记录了190个人的标记数据用于地图构建。发现大多数标记是共显性的，其中八个标记显示出对Shaheen Basmati等位基因有利，而四个标记对Pokkali等位基因则占优势。检出的偏析畸变率为7.47％，明显低于以前的研究。使用MapMaker来构建链接组。该图跨1753.9 cM（Kosambi函数），在12个水稻染色体上带有微卫星标记，标记之间的平均距离为16.2 cM。该图谱分别在连锁组3和12上包含较大和最小百分比的标记。最大共同区间大小（MCIS）分析显示，目前的图谱（印度×稻）覆盖了先前调查的98％的水稻基因组（粳稻）。由于亲本品种背景多样，分离偏低和基因组覆盖率高，表明分子连锁图谱可作为标记框架研究to稻重要农艺性状的遗传。

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