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首页> 外文期刊>Pathogens >Exploring PFGE for Detecting Large Plasmids in Campylobacter jejuni and Campylobacter coli Isolated from Various Retail Meats
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Exploring PFGE for Detecting Large Plasmids in Campylobacter jejuni and Campylobacter coli Isolated from Various Retail Meats

机译：探索用于检测从各种零售肉中分离出的空肠弯曲杆菌和大肠杆菌弯曲杆菌中大型质粒的PFGE

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Campylobacter spp. is one of the most prevalent bacterial pathogens in retail meat, particularly poultry, and is a leading cause of diarrhea in humans. Studies related to Campylobacter large plasmids are limited in the literature possibly due to difficulty in isolating them using available alkaline lysis methods. The objectives of this study were to determine the prevalence of plasmids, particularly large ones, in Campylobacter spp. isolated from various Oklahoma retail meats, and to explore PFGE (Pulsed Field Gel Electrophoresis) as a tool in facilitating the detection of these plasmids. One hundred and eighty nine strains (94 Campylobacter jejuni and 95 Campylobacter coli) were screened for the presence of plasmids using both alkaline lysis and PFGE. Plasmids were detected in 119/189 (63%) using both methods. Most of the plasmids detected by alkaline lysis were smaller than 90 kb and only three were larger than 90 kb. Plasmids over 70 kb in size were detected in 33 more strains by PFGE of which 11 strains contained larger than 90 kb plasmids. Plasmids were more prevalent in Campylobacter coli (73.5%) than in Campylobacter jejuni (52%). BglII restriction analysis of plasmids isolated from 102 isolates revealed 42 different restriction patterns. In conclusion, PFGE was able to detect large plasmids up to 180 Kb in Campylobacter spp. which might have been missed if the alkaline lysis method was solely used. Campylobacter spp. isolated from retail meats harbor a diverse population of plasmids with variable sizes. To our knowledge, this is the first study to use PFGE to detect large plasmids in Campylobacter.
机译：弯曲杆菌属。它是零售肉类（尤其是家禽）中最普遍的细菌病原体之一，并且是导致人类腹泻的主要原因。与弯曲杆菌大质粒相关的研究在文献中受到限制，这可能是由于使用现有的碱性裂解方法难以分离它们所致。这项研究的目的是确定弯曲杆菌属中质粒，特别是大质粒的普遍性。分离自俄克拉荷马州各种零售肉类，并探索PFGE（脉冲场凝胶电泳）作为促进检测这些质粒的工具。使用碱性裂解法和PFGE法筛选了189个菌株（空肠弯曲杆菌94和大肠弯曲杆菌95）是否存在质粒。使用两种方法在119/189（63％）中检测到质粒。通过碱裂解检测到的大多数质粒小于90 kb，只有三个大于90 kb。通过PFGE在33多个菌株中检测到大小超过70 kb的质粒，其中11个菌株含有大于90 kb的质粒。质粒在空肠弯曲杆菌（73.5％）比空肠弯曲杆菌（52％）更普遍。从102个分离物中分离的质粒的BglII限制性酶切分析揭示了42种不同的限制性酶切模式。总之，PFGE能够在弯曲杆菌属中检测到最大180 Kb的大质粒。如果仅使用碱性裂解方法，则可能会漏掉它。弯曲杆菌属。从零售肉中分离出的质粒包含了各种大小可变的质粒。据我们所知，这是第一项使用PFGE检测弯曲杆菌中大质粒的研究。

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