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Establishment of a continuous culture system for Entamoeba muris and analysis of the small subunit rRNA gene

机译：建立鼠疫肠杆菌的连续培养系统并分析小亚基rRNA基因

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We established a culture system for Entamoeba muris (MG-EM-01 strain isolated from a Mongolian gerbil) using a modified Balamuth’s egg yolk infusion medium supplemented with 4% adult bovine serum and Bacteroides fragilis cocultured with Escherichia coli. Further, encystation was observed in the culture medium. The morphological characteristics of E. muris are similar to those of Entamoeba coli (E. coli); moreover, the malic isoenzyme electrophoretic band, which shows species-specific electrophoretic mobility, of E. muris had almost the same mobility as that observed with the malic isoenzyme electrophorectic band of E. coli (UZG-EC-01 strain isolated from a gorilla). We determined the small subunit rRNA (SSU-rRNA) gene sequence of the MG-EM-01 strain, and this sequence was observed to show 82.7% homology with that of the UZG-EC-01 strain. Further, the resultant phylogenetic tree for molecular taxonomy based on the SSU-rRNA genes of the 21 strains of the intestinal parasitic amoeba species indicated that the MG-EM-01 strain was most closely related to E. coli.
机译：我们使用改良的Balamuth蛋黄注入培养基，添加了4％的成年牛血清和与大肠杆菌共培养的脆弱拟杆菌，建立了鼠疫杆菌（从蒙古沙鼠中分离出的MG-EM-01菌株）的培养系统。此外，在培养基中观察到渗入。鼠埃里希氏菌的形态学特征与Entemoeba coli（E. coli）相似。此外，显示出物种特异性电泳迁移率的大肠杆菌的苹果同工酶电泳带具有与大肠杆菌（从大猩猩分离的UZG-EC-01菌株）的苹果同工酶电泳带观察到的几乎相同的迁移率。 。我们确定了MG-EM-01菌株的小亚基rRNA（SSU-rRNA）基因序列，并观察到该序列与UZG-EC-01菌株具有82.7％的同源性。此外，基于21种肠道寄生变形虫种类的SSU-rRNA基因的分子分类学系统发育树表明，MG-EM-01菌株与大肠杆菌最密切相关。

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