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首页> 外文期刊>Parasites Vectors >Anopheles sinensis mosquito insecticide resistance: comparison of three mosquito sample collection and preparation methods and mosquito age in resistance measurements
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Anopheles sinensis mosquito insecticide resistance: comparison of three mosquito sample collection and preparation methods and mosquito age in resistance measurements

机译：中华按蚊蚊虫杀虫剂抗性：三种蚊虫样品采集和制备方法及蚊虫年龄的抗药性比较

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Background Insecticide resistance monitoring in malaria mosquitoes is essential for guiding the rational use of insecticides in vector control programs. Resistance bioassay is the first step for insecticide monitoring and it lays an important foundation for molecular examination of resistance mechanisms. In the literature, various mosquito sample collection and preparation methods have been used, but how mosquito sample collection and preparation methods affect insecticide susceptibility bioassay results is largely unknown. The objectives of this study were to determine whether mosquito sample collection and preparation methods affected bioassay results, which may cause incorrect classification of mosquito resistance status. Methods The study was conducted in Anopheles sinensis mosquitoes in two study sites in central China. Three mosquito sample collection and preparation methods were compared for insecticide susceptibility, kdr frequencies and metabolic enzyme activities: 1) adult mosquitoes collected from the field; 2) F1 adults from field collected, blood-fed mosquitoes; and 3) adult mosquitoes reared from field collected larvae. Results Mosquito sample collection and preparation methods significantly affected mortality rates in the standard WHO tube resistance bioassay. Mortality rate of field-collected female adults was 10-15% higher than in mosquitoes reared from field-collected larvae and F1 adults from field collected blood-fed females. This pattern was consistent in mosquitoes from the two study sites. High kdr mutation frequency (85-95%) with L1014F allele as the predominant mutation was found in our study populations. Field-collected female adults consistently exhibited the highest monooxygenase and GST activities. The higher mortality rate observed in the field-collected female mosquitoes may have been caused by a mixture of mosquitoes of different ages, as older mosquitoes were more susceptible to deltamethrin than younger mosquitoes. Conclusions Female adults reared from field-collected larvae in resistance bioassays are recommended to minimize the effect of confounding factors such as mosquito age and blood feeding status so that more reliable and reproducible mortality may be obtained.
机译：背景技术监测疟疾蚊子中的杀虫剂抗药性对于指导在媒介控制程序中合理使用杀虫剂至关重要。抗药性生物测定是监测杀虫剂的第一步，它为抗药性机理的分子检查奠定了重要基础。在文献中，已经使用了各种蚊子样品的收集和制备方法，但是蚊子样品的收集和制备方法如何影响杀虫剂敏感性生物测定结果尚不清楚。这项研究的目的是确定蚊子样本的收集和制备方法是否会影响生物测定结果，从而可能导致对蚊子抗药性状态的错误分类。方法在中国中部两个研究地点的中华按蚊中进行研究。比较了三种蚊虫样品的采集和制备方法的杀虫剂敏感性，kdr频率和代谢酶活性：1）从现场采集的成年蚊子; 2）现场采集的F1成年人通过血养蚊子采集; 3）从田间饲养的成年蚊子收集幼虫。结果在标准的WHO管抗性生物测定中，蚊子样品的收集和制备方法显着影响死亡率。田间采集的成年女性的死亡率比田间采集的幼虫和外来采血的雌性的F1成虫的死亡率高10-15％。在两个研究地点的蚊子中，这种模式是一致的。在我们的研究人群中发现了以L1014F等位基因为主要突变的高kdr突变频率（85-95％）。现场收集的成年女性始终表现出最高的单加氧酶和GST活性。在野外采集的雌性蚊子中观察到的较高死亡率可能是由于不同年龄的蚊子混合造成的，因为年龄较大的蚊子比年龄较小的蚊子更容易受到溴氰菊酯的影响。结论建议在抵抗力生物测定法中从田间幼虫饲养的成年雌性动物尽量减少混杂因素（例如蚊龄和采血状况）的影响，以便获得更可靠和可再现的死亡率。

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