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首页> 外文期刊>Sensing and Bio-Sensing Research >Clinical diagnosis of type I allergy by means of {SPR} imaging with less than a microliter of peripheral blood
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Clinical diagnosis of type I allergy by means of {SPR} imaging with less than a microliter of peripheral blood

机译：不到一微升外周血的{SPR}成像对I型过敏的临床诊断

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Abstract The identification of antigen that induces activation of mast cells and basophils by crosslinking IgE bound to the cell surface is crucial to avoid symptoms of allergic diseases. Surface plasmon resonance imaging (SPRI) possesses a great potential for clinical diagnosis of allergy, in that it reveals living cell activation following the binding of antigens to IgE, on real-time and single cell basis without artificial labeling. However, present technique of {SPRI} requires freshly isolated basophils of patients and cannot analyze multiple samples in parallel. To overcome such problems, we developed devices for {SPRI} to make a broad observation area and a multi-well {SPRI} sensor chip with a hydrophobic membrane. The employment of human IgE receptor-expressing mast cell lines (RBL-48 cells) sensitized with serum, collected and stored from less than a microliter of patient’s blood, allowed us to detect specific reactions of RBL-48 cells in response to antigens. This technique may be a useful tool as a high throughput screening system of type I allergy not only for freshly prepared basophils but also for sera stored in clinical practices.
机译：摘要鉴定能通过使结合在细胞表面的IgE交联来诱导肥大细胞和嗜碱性粒细胞活化的抗原，对于避免过敏性疾病的症状至关重要。表面等离振子共振成像（SPRI）在过敏的临床诊断中具有巨大潜力，因为它揭示了抗原与IgE结合后在实时和单细胞基础上的活细胞激活，无需人工标记。然而，{SPRI}的现有技术需要新鲜分离的患者嗜碱性粒细胞，并且不能并行分析多个样品。为了克服这些问题，我们开发了{SPRI}的设备，使其具有广阔的观察范围，并开发了带有疏水膜的多井{SPRI}传感器芯片。使用从血清中致敏的人类IgE受体表达肥大细胞系（RBL-48细胞），从不到一微升的患者血液中收集和储存，使我们能够检测RBL-48细胞对抗原的特异性反应。该技术可能是有用的工具，不仅对于新鲜制备的嗜碱性粒细胞，而且对于临床实践中存储的血清，都是I型过敏的高通量筛选系统。

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