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首页> 外文期刊>Pharmacognosy Research >Identification of the protease inhibitor miraziridine A in the Red sea sponge Theonella swinhoei
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Identification of the protease inhibitor miraziridine A in the Red sea sponge Theonella swinhoei

机译：红海海绵Theonella swinhoei中蛋白酶抑制剂miraziridine A的鉴定

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Background:Miraziridine A, a natural peptide isolated from a marine sponge, is a potent cathepsin B inhibitor with a second-order rate constant of 1.5 × 104 M-1 s-1. In the present study, miraziridine A was isolated from the Red Sea sponge Theonella swinhoei on the basis of chromatographic and spectrometric techniques. We conclude that T. swinhoei from the Red Sea represents an alternative source of the aziridinylpeptide miraziridine A to the previously identified Theonella mirabilis from Japan. We confirmed that the metabolite is produced by marine sponges from different geographical locations.Context:Marine sponges have been proven to be a rich source of secondary metabolites exhibiting a huge diversity of biological activities, including antimicrobial, antitumor and immunomodulatory activities. Theonella species (order Lithistida, Demospongiae) have been shown to be a source of anti-protease and anti-HIV secondary metabolites.Aims:To identify the protease inhibitor mirazirine A in the marine sponge Theonella swinhoei.Material and Methods:The marine sponge Theonella swinhoei was collected by SCUBA diving in the Red Sea in Eilat (Israel). Sponge material was lyophilized and further extracted successively with cyclohexane, dichloromethane and methanol to obtain three crude extracts. LC-MS analysis was performed to confirm the presence of Miraziridine A in the dichloromethane fraction.Results:In the present study, miraziridine A was isolated from the Red Sea sponge T. swinhoei on the basis of chromatographic and spectrophotometric techniques.Conclusions:We conclude that T. swinhoei from the Red Sea represents an alternative source of the aziridinylpeptide miraziridine A to the previously identified Theonella mirabilis from Japan.
机译：背景：美拉西啶A是一种从海洋海绵中分离的天然肽，是一种有效的组织蛋白酶B抑制剂，其二级速率常数为1.5×104 M-1 s-1。在本研究中，根据色谱和光谱技术从红海海绵Theonella swinhoei中分离了咪拉西啶A。我们得出的结论是，来自红海的T. swinhoei代表了氮丙啶基肽米拉西立啶A的替代来源，这是先前确定的来自日本的奇异菌小提琴。我们确认了代谢产物是由来自不同地理位置的海洋海绵产生的。背景：海洋海绵已被证明是次生代谢产物的丰富来源，其具有多种多样的生物活性，包括抗微生物，抗肿瘤和免疫调节活性。茶色菌（Lithistida，Demospongiae）是抗蛋白酶和抗HIV次生代谢产物的来源。 swinhoei是由SCUBA潜水在以色列埃拉特（Eilat）的红海中采集的。将海绵材料冻干，并进一步依次用环己烷，二氯甲烷和甲醇萃取，得到三种粗提物。结果：本研究采用色谱和分光光度法从红海海绵T. swinhoei中分离了咪拉西啶A。认为来自红海的斯温霍伊猪笼草是叠氮基腺苷肽咪拉西啶A的替代来源，是先前鉴定的日本提奥奈拉菌的替代来源。

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