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首页> 外文期刊>Pharmacognosy Research >A validated UV-HPLC method for determination of chlorogenic acid in Lepidogrammitis drymoglossoides (Baker) Ching, Polypodiaceae
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A validated UV-HPLC method for determination of chlorogenic acid in Lepidogrammitis drymoglossoides (Baker) Ching, Polypodiaceae

机译：一种经过验证的UV-HPLC方法，用于测定Poly足科的干鳞状湿疹（贝克）中的绿原酸

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Background:Lepidogrammitis drymoglossoides (Baker) Ching (L. drymoglossoides), a member of the Polypodiaceae family, was used in the treatment of numerous diseases. However, none of the potential ingredients and the quality control methods concerning this plant medicine was pronounced.Objective:To identify chlorogenic acid (CGA) from L. drymoglossoides and develop a high performance liquid chromatography (HPLC) assay of CGA.Materials and Methods:UV, TLC, and HPLC were utilized to identify the phytochemicals of L. drymoglossoides and determine the CGA content, respectively. The HPLC conditions were as following: a Phenomenex Luna C18 (2) (250 × 4.6 mm i.d.; 5 μm particle size; 100 ? pore size) column; the mobile phase of the mixture of acetonitrile and 0.5% aqueous phosphoric acid (11.5:88.5 v/v); the flow rate of 1.0 mL/min and determination wavelength of 327 nm.Results:The proposed HPLC method has been developed and validated. The calibration curve was y = 28328x + 16610 (R2 = 0.9997). The intra-day and inter-day precision and intermediate precision were validated with the RSD less than 5%. The mean recovery rate of the method ranged from 95% to 104%, with the RSD less than 5%. The LOD and LQD values were 0.049 and 0.132 mg/L, respectively. The content of CGA in L. drymoglossoides approximately reached 0.24% (v/v) by the proposed extraction and determination methods.Conclusion:The assay method was simple, convenient, and accurate to the quantification of CGA and can be used for the quality control of the herb.
机译：背景：淋病性干眼病（贝克）Ching（L. drymoglossoides）是Poly科的一员，被用于治疗多种疾病。然而，尚无与该植物药有关的潜在成分和质量控制方法的任何信息。目的：从干乳杆菌中鉴定绿原酸（CGA），并开发一种高效液相色谱（HPLC）测定CGA的方法和材料：利用紫外线，薄层色谱和高效液相色谱分别鉴定干珠菌的植物化学成分并确定CGA含量。 HPLC条件如下：Phenomenex Luna C18（2）（250×4.6mm i.d.;5μm粒径;100μm孔径）色谱柱;色谱柱。乙腈和0.5％磷酸水溶液（11.5：88.5 v / v）的混合物的流动相;结果：所建立的HPLC方法得到了验证。校准曲线为y = 28328x + 16610（R2 = 0.9997）。日内和日间精度和中间精度在RSD小于5％的情况下得到验证。该方法的平均回收率在95％至104％之间，RSD小于5％。 LOD和LQD值分别为0.049和0.132 mg / L。通过所提出的提取和测定方法，干珠菌中CGA的含量约为0.24％（v / v）。结论：该测定方法简便，方便，准确，可用于CGA的定量，可用于质量控制的草药。

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