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首页> 外文期刊>Pharmacognosy Research >Black tea (Camellia sinensis) decoction shows immunomodulatory properties on an experimental animal model and in human peripheral mononuclear cells
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Black tea (Camellia sinensis) decoction shows immunomodulatory properties on an experimental animal model and in human peripheral mononuclear cells

机译：红茶汤对实验动物模型和人外周血单个核细胞具有免疫调节作用

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Background:Black tea (Camellia sinensis) has been used as a daily beverage for time immemorial. Immunomodulatory effects of tea are recognized as it stimulates the proliferation of cultured human peripheral blood mononuclear cells. Anti-inflammatory effects of tea have also been depicted in the available literature. Therefore, we designed this study to examine the potential immunemodulatory and anti-inflammatory activities of black tea in a rat model and in human peripheral mononuclear cells.Aims:The purpose of the study was to determine (1) evaluation of anti-inflammatory effects of black tea on rats, (2) evaluation of immunemodulator effects of black tea on rats, and (3) evaluation of immunemodulator effects of black tea on human peripheral mononuclear cells.Materials and Methods:Black tea decoction (10% and 20%) was prepared. Acute anti-inflammatory activity of tea decoction was evaluated using carrageenan and dextran whereas chronic anti-inflammatory (Immunomodulatory) effects were evaluated in a complete Freunds’ adjuvant-induced arthritis model. Immunostimulatory role was evaluated in cultured human (in vitro) peripheral mononuclear cells (T-lymphocytes) by using methyl thioazolyl tetrazolium (MTT) and Trypan blue assay.Study Design:An experimental study was designed.Results:Black tea decoction (10% and 20%) strength has shown significant anti-inflammatory effects (64.8% and 77% reduction, respectively), on carrageenan-induced acute inflammatory models (rat paw edema) which can be comparable with the standard drug indomethacin (89.1%). In a chronic anti-inflammatory model, black tea decoction (10% and 20%) has shown significant suppressive effects on rat paw edema (38.56% and 69.53%) observed on 21st day. Lymphoproliferative action of tea was evaluated on human peripheral mononuclear cells using an MTT assay where the number of living cells were expressed in terms of optical density at 570 nm. An experiment has shown that black tea increases the maximum number of T-lymphocytes at 72 h with a maximum strength of 20%. Maximum number of viable cells (T-lymphocytes) was observed with black tea at 20% strength at 72 h. The results were expressed as mean ± SD, and the significance was evaluated by Student's t-test versus control, with P < 0.05 implying significance.Conclusions:Taken together, our data indicate that black tea has potential anti-inflammatory and immunomodulatory action and this corroborates with the current trend of tea being promoted as a ‘health drink’.
机译：背景：红茶（Camellia sinensis）已被用作纪念时间的日常饮料。茶的免疫调节作用是公认的，因为它可以刺激培养的人外周血单核细胞的增殖。茶的抗炎作用也已在现有文献中描述。因此，我们设计了这项研究，以研究红茶在大鼠模型和人外周血单个核细胞中的潜在免疫调节和抗炎活性。目的：本研究的目的是确定（1）评估红茶的抗炎作用红茶对大鼠的影响;（2）红茶对大鼠的免疫调节作用的评估;（3）红茶对人外周血单个核细胞的免疫调节作用的评价。材料与方法：红茶汤（分别为10％和20％）准备好了。使用角叉菜胶和葡聚糖评估茶汤的急性抗炎活性，而在完整的弗氏佐剂诱发的关节炎模型中评估慢性抗炎（免疫调节）作用。采用甲基噻唑基四唑鎓（MTT）和锥虫蓝试验评估了培养的人（体外）外周血单个核细胞（T淋巴细胞）的免疫刺激作用。研究设计：实验研究结果：红茶汤（10％ 20％的强度对角叉菜胶诱导的急性炎症模型（大鼠爪水肿）显示出显着的抗炎作用（分别降低64.8％和77％），可与标准药物消炎痛（89.1％）相媲美。在慢性消炎模型中，在第21天观察到的红茶汤（10％和20％）对大鼠爪水肿具有显着的抑制作用（38.56％和69.53％）。使用MTT测定法评估了茶对人外周单核细胞的淋巴增生作用，其中活细胞的数量以570 nm处的光密度表示。实验表明，红茶在72小时内增加了T淋巴细胞的最大数量，最大强度为20％。在72 h时，用浓度为20％的红茶观察到最大数量的活细胞（T淋巴细胞）。结果表示为平均值±SD，并通过Student's t检验与对照进行显着性评估，P <0.05表示显着性。结论：综上所述，我们的数据表明红茶具有潜在的抗炎和免疫调节作用，并且证实了当前茶被推广为“健康饮料”的趋势。

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