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Phytochemical Screening and Antimicrobial Properties of a Common Brand of Black Tea (Camellia sinensis) Marketed in Nigerian Environment

机译:在尼日利亚环境中销售的普通品牌红茶(山茶)的植物化学筛选和抗菌特性

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摘要

>Purpose: This study is aimed at determining chemical constituents and antimicrobial activities of a common brand of black tea (Lipton®) in Nigeria. >Method: Standard methods were employed for testing carbohydrates, tannins, saponins, flavonoids, alkaloids, steroids and terpenes in the tea. Antimicrobial activities of methanolic and aqueous extracts of the tea on four standard strains of organisms: Escherichia coli, Pseudomonas aeruginosa, Staphylococcus aureus and Bacillus subtilis were also determined by standard methods. >Result: Results showed that the tea contains tannin and reducing sugar. Concentrations of 1%, 2%, 4%, 6%, 8% and 10% aqueous and methanolic extract of black tea were prepared and their zones of inhibition determined against the four test organisms using the cup plate method. This was compared with zones for standard disc Gentamicin (10 µg) and Erythromycin (15 µg). Pseudomonas aeruginosa was sensitive to 2% to 10% aqueous extracts and intermediate to 6%, 8% and 10% methanolic extracts. E. coli was intermediately sensitive to 6%, 8% and 10% aqueous extract and 2% to 10% methanolic extracts. B. subtilis was intermediately sensitive to 4%, 6% and 8% aqueous extract and 4% to 10% methanolic extract but sensitive to 10% aqueous extract. Staph.aureus was intermediately sensitive to 4% to 10% aqueous extracts and 2% to 10% methanolic extracts. B. subtilis had the lowest MIC values of both aqueous and methanolic extracts. In conclusion, this study has shown that Lipton® has antimicrobial properties on E.coli, Staph.aureus, B.subtilis and Ps.aeruginosa and contains tannin and reducing sugar.
机译:>目的:本研究旨在确定尼日利亚一个常见品牌的红茶(Lipton®)的化学成分和抗菌活性。 >方法:采用标准方法测试茶中的碳水化合物,单宁,皂角苷,类黄酮,生物碱,类固醇和萜烯。还通过标准方法测定了茶的甲醇提取物和水提取物对四种标准生物体的抗菌活性:大肠杆菌,铜绿假单胞菌,金黄色葡萄球菌和枯草芽孢杆菌。 >结果:结果表明该茶含有单宁和还原糖。制备浓度分别为1%,2%,4%,6%,8%和10%的红茶水和甲醇提取物,并使用杯板法确定其对四种测试生物的抑制区域。将其与标准盘庆大霉素(10 µg)和红霉素(15 µg)的区域进行比较。铜绿假单胞菌对2%至10%的水提取物敏感,对6%,8%和10%的甲醇提取物敏感。大肠杆菌对6%,8%和10%的水提取物以及2%至10%的甲醇提取物具有中等敏感性。枯草芽孢杆菌对4%,6%和8%的水提取物和4%至10%的甲醇提取物中等敏感,但对10%的水提取物敏感。金黄色葡萄球菌对4%至10%的水提取物和2%至10%的甲醇提取物中等敏感。枯草芽孢杆菌在含水和甲醇提取物中的最低MIC值。总之,这项研究表明,Lipton®对大肠杆菌,金黄色葡萄球菌,枯草芽孢杆菌和铜绿假单胞菌具有抗菌性能,并且含有单宁和还原糖。

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