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首页> 外文期刊>Physiological Reports >Blunted hypothalamic ghrelin signaling reduces diet intake in rats fed a low‐protein diet in late pregnancy
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Blunted hypothalamic ghrelin signaling reduces diet intake in rats fed a low‐protein diet in late pregnancy

机译：下丘脑促生长素释放肽信号钝化可降低妊娠晚期低蛋白饮食大鼠的饮食摄入

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AbstractDiet intake in pregnant rats fed a low-protein (LP) diet was significantly reduced during late pregnancy despite elevated plasma levels of ghrelin. In this study, we hypothesized that ghrelin signaling in the hypothalamus is blunted under a low-protein diet condition and therefore, it does not stimulate diet intake during late pregnancy. Female Sprague–Dawley rats were fed a normal (CT) or LP diet from Day 1 of pregnancy. On Day 21, 0.5 μg ghrelin was given into the third ventricle (ICV). Diet and water intake at 30, 60, and 120 min after ICV injection was measured. Hypothalami were dissected and analyzed for expression of genes related to appetite regulation (Npy, Agrp, Pomc and Cart) and phosphorylation of AMPK and ACC proteins (downstream proteins of ghrelin receptor activation). Results include: In response to ICV injection of ghrelin, (1) diet intake was significantly lower in LP compared to CT rats; (2) water intake was not affected in LP rats; (3) expression of Npy and Agrp, but not Pomc and Cart, were higher in the hypothalamus of LP compared to CT rats; (4) the abundance of phosphorylated AMPK and the ratio of phosphorylated to total AMPK, but not the abundance of total AMPK, were lower in LP compared to CT rats; (5) the abundance of phosphorylated ACC, but not total ACC, was lower in LP rats. These findings suggest that blunted ghrelin signaling in the hypothalamus of pregnant rats fed a LP diet leads to reduced diet intake and exacerbates gestational protein insufficiency.
机译：摘要尽管生长激素释放肽的血浆水平升高，但在妊娠后期，低蛋白饮食的孕妇的饮食摄入量显着降低。在这项研究中，我们假设在低蛋白饮食条件下下丘脑中的生长素释放肽信号传导减弱，因此，它不会在妊娠后期刺激饮食摄入。从怀孕的第一天开始，对雌性Sprague–Dawley大鼠进行正常（CT）或LP饮食喂养。在第21天，将0.5μgghrelin注入第三脑室（ICV）。注射ICV后30、60和120分钟测量饮食和水摄入量。解剖下丘脑并分析与食欲调节相关的基因表达（Npy，Agrp，Pomc和Cart）以及AMPK和ACC蛋白（ghrelin受体激活的下游蛋白）的磷酸化。结果包括：针对ICV注射生长激素释放肽，（1）与CT大鼠相比，LP的饮食摄入量显着降低; （2）LP大鼠的饮水量不受影响; （3）LP下丘脑中的Npy和Agrp的表达高于CT大鼠，而Pomc和Cart的则没有。 （4）与CT大鼠相比，LP中磷酸化AMPK的丰度和磷酸化与总AMPK的比率降低，但不降低总AMPK的丰度。 （5）LP大鼠中磷酸化ACC的丰度较低，而不是总ACC较低。这些发现表明，接受低脂饮食的怀孕大鼠下丘脑中生长素释放肽信号的减弱导致饮食摄入减少，并加剧了妊娠蛋白的不足。

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